Anti-NOXA1 抗体 (ab68523)
Key features and details
- Mouse polyclonal to NOXA1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-NOXA1 antibody
NOXA1 一次抗体 製品一覧 -
製品の詳細
Mouse polyclonal to NOXA1 -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein within Human NOXA1. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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ポジティブ・コントロール
- NOXA1 transfected 293T lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab68523の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 51 kDa).
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特記事項 |
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WB
1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 51 kDa). |
ターゲット情報
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機能
Functions as an activator of NOX1, a superoxide-producing NADPH oxidase. Functions in the production of reactive oxygen species (ROS) which participate in a variety of biological processes including host defense, hormone biosynthesis, oxygen sensing and signal transduction. May also activate CYBB/gp91phox and NOX3. -
組織特異性
Widely expressed. Detected in pancreas, liver, kidney, spleen, prostate, small intestine and colon. -
配列類似性
Belongs to the NCF2/NOXA1 family.
Contains 1 OPR domain.
Contains 1 SH3 domain.
Contains 4 TPR repeats. -
発生段階
Expressed in fetal kidney. -
ドメイン
The SH3 domain mediates interaction with NOXO1 and NCF1 and has autoregulatory function.
The TPR repeats mediate interaction with RAC1. -
翻訳後修飾
Interaction with YWHAZ depends on phosphorylation by PKA. -
細胞内局在
Cytoplasm. Cell membrane. Translocation to membranes depends on NOXO1 or NCF1 and maybe RAC1. - Information by UniProt
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参照データベース
- Entrez Gene: 10811 Human
- Omim: 611255 Human
- SwissProt: Q86UR1 Human
- Unigene: 495554 Human
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別名
- Antigen NY CO 31 antibody
- Antigen NY-CO-31 antibody
- FLJ25475 antibody
see all
画像
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All lanes : Anti-NOXA1 antibody (ab68523) at 1/500 dilution
Lane 1 : NOXA1 transfected 293T lysate
Lane 2 : Non-transfected 293T lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size: 51 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
データシートおよび資料
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Datasheet download
参考文献 (5)
ab68523 は 5 報の論文で使用されています。
- De Bessa TC et al. Subverted regulation of Nox1 NADPH oxidase-dependent oxidant generation by protein disulfide isomerase A1 in colon carcinoma cells with overactivated KRas. Cell Death Dis 10:143 (2019). PubMed: 30760703
- Chocry M et al. Reversion of resistance to oxaliplatin by inhibition of p38 MAPK in colorectal cancer cell lines: involvement of the calpain / Nox1 pathway. Oncotarget 8:103710-103730 (2017). PubMed: 29262595
- Streeter J et al. Phosphorylation of Nox1 regulates association with NoxA1 activation domain. Circ Res 115:911-8 (2014). PubMed: 25228390
- Csányi G et al. Nox2 B-loop peptide, Nox2ds, specifically inhibits the NADPH oxidase Nox2. Free Radic Biol Med 51:1116-25 (2011). WB ; Human . PubMed: 21586323
- Biasi F et al. Pro-oxidant and proapoptotic effects of cholesterol oxidation products on human colonic epithelial cells: a potential mechanism of inflammatory bowel disease progression. Free Radic Biol Med 47:1731-41 (2009). IP ; Human . PubMed: 19778604