製品の概要

  • 製品名Anti-Noxa antibody
    Noxa 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Noxa
  • 特異性Although immunogen is from Mouse, customer feedback indicates that the results for Mouse are non-specific (see Abreviews).
  • アプリケーション適用あり: WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rat
  • 免疫原

    Synthetic peptide corresponding to 17 amino acids at the amino terminus of mouse Noxa. (Peptide available as ab39823.)

  • ポジティブ・コントロール
    • Human stomach tissue lysate.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab36833 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 11.6 (mouse), 6 (human) kDa.Can be blocked with Human Noxa peptide (ab39823).
IHC-P Use a concentration of 1 µg/ml.
ICC/IF Use a concentration of 1 - 5 µg/ml.

ターゲット情報

  • 機能Promotes activation of caspases and apoptosis. Promotes mitochondrial membrane changes and efflux of apoptogenic proteins from the mitochondria. Contributes to p53/TP53-dependent apoptosis after radiation exposure. Promotes proteasomal degradation of MCL1. Competes with BAK1 for binding to MCL1 and can displace BAK1 from its binding site on MCL1 (By similarity). Competes with BIM/BCL2L11 for binding to MCL1 and can displace BIM/BCL2L11 from its binding site on MCL1.
  • 組織特異性Highly expressed in adult T-cell leukemia cell line.
  • 配列類似性Belongs to the PMAIP1 family.
  • ドメインThe BH3 motif is essential for pro-apoptotic activity.
  • 細胞内局在Mitochondrion.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Adult T cell leukemia derived PMA responsive antibody
    • APR antibody
    • APR_HUMAN antibody
    • ATL-derived antibody
    • Immediate early response protein APR antibody
    • Immediate-early-response protein APR antibody
    • NOXA antibody
    • Phorbol 12 myristate 13 acetate induced protein 1 antibody
    • Phorbol-12-myristate-13-acetate-induced protein 1 antibody
    • PMA induced protein 1 antibody
    • PMA-induced protein 1 antibody
    • PMA-responsive gene antibody
    • Pmaip1 antibody
    • Protein Noxa antibody
    see all

Anti-Noxa antibody 画像

  • Anti-Noxa antibody (ab36833) at 0.5 µg/ml + Human stomach cell lysate

    Predicted band size : 11.6 (mouse), 6 (human) kDa
    Observed band size : 15 kDa (why is the actual band size different from the predicted?)
  • ab36833 at 1µg/ml staining Noxa in human stomach tissue by IHC

  • ICC/IF image of ab36833 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab36833, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immunofluorescence of Noxa in Human Stomach cells using ab36833 at 10 ug/ml.

Anti-Noxa antibody (ab36833) 使用論文

This product has been referenced in:
  • Thomas SE  et al. p53 and translation attenuation regulate distinct cell cycle checkpoints during endoplasmic reticulum (ER) stress. J Biol Chem 288:7606-17 (2013). WB ; Human . Read more (PubMed: 23341460) »
  • Marshall AD  et al. PAX3-FOXO1 induces up-regulation of Noxa sensitizing alveolar rhabdomyosarcoma cells to apoptosis. Neoplasia 15:738-48 (2013). WB . Read more (PubMed: 23814486) »

See all 7 Publications for this product

Product Wall

Thank you for contacting us. We do not have any recommendation for further optimization. I would like to ask, how you would like to proceed? Would you like to have same antibody from different lot or want refund of your purchase?

Thank you for your enquiry regarding ab36833 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody. Additionally, thank you for supplying an image, as this has...

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Application Western blot
Sample Mouse Recombinant protein (Recombinant)
Loading amount 0.05 µg
Specification Recombinant
Gel Running Conditions Reduced Denaturing (10% Bis-Tris)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Dec 04 2009

Application Western blot
Sample Human Cell lysate - whole cell (multiple myeloma (RPMI8226) and T-cell leukemia (J)
Loading amount 30 µg
Specification multiple myeloma (RPMI8226) and T-cell leukemia (J
Treatment Velcade for 18 h
Blocking step Milk as blocking agent for 10 hour(s) and 0 minute(s) · Concentration: 5%
Username

Dr. Isabel Marzo

Verified customer

投稿 Aug 01 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"