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Our Abpromise guarantee covers the use of ab80508 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|Flow Cyt||Use at an assay dependent concentration.|
|IHC-Fr||Use a concentration of 5 µg/ml.|
|IHC-P||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).Can be blocked with Human NOX2/gp91phox peptide (ab111671).
Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
As published in databases Human Protein Atlas, GeneCard and NCBI CYBB expression in heart is cell type specific therefore, we recommend including positive controls when using this antibody with heart samples.
Primary incubated for 9 hours at 4°C.