製品の概要

  • 製品名Anti-NOX2/gp91phox antibody
    NOX2/gp91phox 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to NOX2/gp91phox
  • アプリケーション適用あり: IHC-P, ICC/IF, WBmore details
  • 種交差性
    交差種: Rat, Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human NOX2/gp91phox.

    (Peptide available as ab32458.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal against the NOX2 Human Partial Recombinant Protein (Tagged) and Rat Liver Tissue Lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab31092 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/2000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration. PubMed: 22184405
WB Use a concentration of 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).

ターゲット情報

  • 機能Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc.
  • 関連疾患Defects in CYBB are a cause of chronic granulomatous disease X-linked (XCGD) [MIM:306400]. Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections.
  • 配列類似性Contains 1 FAD-binding FR-type domain.
    Contains 1 ferric oxidoreductase domain.
  • 翻訳後修飾Glycosylated.
  • 細胞内局在Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AMCBX2 antibody
    • CGD antibody
    • CGD91-phox antibody
    • CY24B_HUMAN antibody
    • CYBB antibody
    • Cytochrome b 245, beta polypeptide antibody
    • Cytochrome b(558) beta chain antibody
    • Cytochrome b(558) subunit beta antibody
    • Cytochrome b-245 heavy chain antibody
    • Cytochrome b558 subunit beta antibody
    • GP91 PHOX antibody
    • gp91-1 antibody
    • gp91-phox antibody
    • GP91PHOX antibody
    • Heme-binding membrane glycoprotein gp91phox antibody
    • NADPH oxidase 2 antibody
    • Neutrophil cytochrome b 91 kDa polypeptide antibody
    • NOX2 antibody
    • p22 phagocyte B-cytochrome antibody
    • P91 PHOX antibody
    • p91-PHOX antibody
    • Superoxide-generating NADPH oxidase heavy chain subunit antibody
    see all

Anti-NOX2/gp91phox antibody 画像

  • Anti-NOX2/gp91phox antibody (ab31092) at 1 µg/ml + NOX2 Human Partial Recombinant Protein at 0.1 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 65 kDa
    Observed band size : 14 kDa (why is the actual band size different from the predicted?)
  • Anti-NOX2/gp91phox antibody (ab31092) at 1 µg/ml + Liver (Rat) Tissue Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 65 kDa
    Observed band size : 65 kDa


    Exposure time : 4 minutes
  • ab31092 (1/2000) staining NOX2/gp91phox in paraffin-embedded Human tonsil tissue. Tissue underwent fixation in formaldehyde, peroxidase blocking, protein blocking and heat mediated antigen retrieval. The secondary antibody was goat anti rabbit conjugated to HRP. For further experimental details please refer to abreview.

    See Abreview

Anti-NOX2/gp91phox antibody (ab31092) 使用論文

This product has been referenced in:
  • Wang C  et al. Metabolic syndrome-induced tubulointerstitial injury: Role of oxidative stress and preventive effects of acetaminophen. Free Radic Biol Med 65C:1417-1426 (2013). Rat . Read more (PubMed: 24140865) »
  • Orcutt KP  et al. Erlotinib-Mediated Inhibition of EGFR Signaling Induces Metabolic Oxidative Stress through NOX4. Cancer Res 71:3932-40 (2011). WB ; Human . Read more (PubMed: 21482679) »

See all 4 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Pig Cell (Cardiomyocyte)
Permeabilization Yes - 0.1% triton x100
Specification Cardiomyocyte
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative Methanol-Acetone & 2%PFA
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投稿 Jul 29 2015

Application Immunocytochemistry/ Immunofluorescence
Sample Cow Cell (aortic endothelial cells)
Permeabilization Yes - Saponin 0,1%
Specification aortic endothelial cells
Blocking step PBS/Saponin 0,1%/BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative PFA 4%
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投稿 Jul 15 2015

Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (dermal fibroblast)
Specification dermal fibroblast
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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投稿 Aug 01 2014

I wanted to answer your question about the two band at different MW.

The difference in MW for the two WBs for ab31092 is due to one
being run on a partial recombinant protein (15 kDa) and the other being run on
endogenous lysates (6...

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DISCOUNT CODE: ABTRIAL-xxxxx
Expiration date: 2013-03-13
Value:€370.00

I am very pleased to hear you would like to accept our offer and test ab31092 in WB and maybe IHC-frozen sections for pig samples. This code will give you &eur...

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Thank you very much for your interest our ab31092 - Anti-NOX2/gp91phox antibody. The antibody is guaranteed for IHC-P, ICC/IF and WB applications.
To our knowledge, the ab31092 has not been tested in pig. For that reason we cannot guarantee it wi...

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Thank you for your phone call.
DISCOUNT CODE: xxx for ab65813
DISCOUNT CODE: xxx for ab31092
DISCOUNT CODE: xxx for ab55831
Expiration date: xxx
I am very pleased to hear you would like to accept our offer and test these 3 anti...

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Thank you for contacting us yesterday in regards to the anti-NOX2/gp91phox antibody (http://www.abcam.com/NOX2-gp91phox-antibody-ab80508.html) which is currently unfortunately out of stock.

As discussed over the phone, I have performed a sequ...

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Pour le http://www.abcam.com/ab31092, j'ai eu la confirmation que la dilution1/2000provient d'un résultat envoyé par un utilisateur et obtenu sur coupes d'amygdale (voir http://www.abcam.com/index.html?datasheet=31092&tab=abreviews&am...

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Merci de votre intérêt pour les anti-NOX2 et anti-NOX4 d'abcam.

L'anti-NOX2 que je recommanderais est le poluclonal de lapinab31092, le lien vers la fiche technique est http://www.abcam.com/ab31092 .

Nous avons 2 anti-N...

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1-10 of 11 Abreviews or Q&A

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