製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to NOX1
  • アプリケーション
    適用あり: ICC/IF, WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Human, Pig
  • 免疫原

    Synthetic peptide corresponding to C terminal residues of human NOX1.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab55831 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
WB Use at an assay dependent concentration. Predicted molecular weight: 65 kDa.Can be blocked with NOX1 peptide (ab194192).
ELISA Use at an assay dependent dilution.

ターゲット情報

  • 機能
    NOH-1S is a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes and other tissues. It participates in the regulation of cellular pH and is blocked by zinc. NOH-1L is a pyridine nucleotide-dependent oxidoreductase that generates superoxide and might conduct H(+) ions as part of its electron transport mechanism, whereas NOH-1S does not contain an electron transport chain.
  • 組織特異性
    NOH-1L is detected in colon, uterus, prostate, and colon carcinoma, but not in peripheral blood leukocytes. NOH-1S is detected only in colon and colon carcinoma cells.
  • 配列類似性
    Contains 1 FAD-binding FR-type domain.
    Contains 1 ferric oxidoreductase domain.
  • 細胞内局在
    Cell projection > invadopodium membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • GP91 2 antibody
    • Mitogenic oxidase (pyridine nucleotide dependent superoxide generating) antibody
    • Mitogenic oxidase 1 antibody
    • MOX-1 antibody
    • MOX1 antibody
    • NADH/NADPH mitogenic oxidase subunit P65 MOX antibody
    • NADH/NADPH mitogenic oxidase subunit P65-MOX antibody
    • NADPH oxidase 1 antibody
    • NADPH oxidase 1 variant NOH 1L antibody
    • NADPH oxidase homolog 1 antibody
    • NOH 1 antibody
    • NOH-1 antibody
    • NOH1 antibody
    • NOX-1 antibody
    • Nox1 antibody
    • NOX1_HUMAN antibody
    • RP1 146H21.1 antibody
    see all

画像

  • ICC/IF image of ab55831 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55831, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

参考文献

This product has been referenced in:
  • Yu P  et al. A dual role of transient receptor potential melastatin 2 channel in cytotoxicity induced by silica nanoparticles. Sci Rep 5:18171 (2015). WB . Read more (PubMed: 26656285) »
  • Van Hée VF  et al. Lactate does not activate NF-?B in oxidative tumor cells. Front Pharmacol 6:228 (2015). WB ; Human . Read more (PubMed: 26528183) »

See all 12 Publications for this product

レビューと Q&A

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Application
Immunoprecipitation
Immuno-precipitation step
Other - Gamma-bind plus protein A/G (half/half)
Sample
Human Cell lysate - whole cell (KERATINOCYTES, Hacat B)
Specification
KERATINOCYTES, Hacat B
Treatment
inhibitor of nox 50´M
Total protein in input
1000 µg
Username

Dr. houssam raad

Verified customer

投稿 Mar 12 2015

Abreviews
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Non-reduced Denaturing (Any Kd)
Sample
Rat Tissue lysate - whole (brain)
Specification
brain
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

投稿 Jun 13 2014

Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Tissue lysate - whole (Liver)
Specification
Liver
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jun 06 2014

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Sample
Human Cell (colon cancer cell)
Specification
colon cancer cell
Permeabilization
Yes - 0.1% Tween 20
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Feb 28 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Liver (non-perfused))
Loading amount
80 µg
Specification
Liver (non-perfused)
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Apr 18 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (WBCs)
Specification
WBCs
Fixative
Acetone
Permeabilization
No
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Mar 25 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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