製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Nova1
  • アプリケーション
    適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Pig, Xenopus laevis
  • 免疫原

    Recombinant protein fragment containing a sequence corresponding to a region within amino acids 315 and 507 of Human Nova1 (NP_002506).

  • ポジティブ・コントロール
    • A431 whole cell lysate

法規制情報

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab97368 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 52 kDa.
ICC/IF 1/100 - 1/200.

ターゲット情報

画像

  • Anti-Nova1 antibody (ab97368) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size : 52 kDa
    7.5% SDS PAGE
  • Immunofluorescence analysis of paraformaldehyde-fixed A431, using Nova1 antibody (ab97368) at 1/200 dilution.

参考文献

This product has been referenced in:
  • Vernia S  et al. An alternative splicing program promotes adipose tissue thermogenesis. Elife 5:N/A (2016). WB ; Mouse . Read more (PubMed: 27635635) »
  • Morchikh M  et al. TOX4 and NOVA1 Proteins Are Partners of the LEDGF PWWP Domain and Affect HIV-1 Replication. PLoS One 8:e81217 (2013). WB, ICC/IF ; Human . Read more (PubMed: 24312278) »

See all 2 Publications for this product

レビューと Q&A

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Sample
Rat Cell (Primary cortical Neuron)
Specification
Primary cortical Neuron
Permeabilization
Yes - PBS-TrironX100 0,1%
Fixative
Paraformaldehyde
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投稿 Mar 25 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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