製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Notch2
  • 特異性
    When tested on human cells overexpressing active Notch 2, this antibody detects a band at about 110kD that represents active Notch 2. This band is not seen in the control cells, confirming that it is Notch 2. The intracellular domain of Notch 2 has a predicted band size of 110kD, corresponding to this band. However, multiple other non-specific bands were also seen in the same experiment.
  • アプリケーション
    適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Peptide:

    C-NAAERTPSHSGHLQ

    aa 2396-2409 (the total protein is 2471 aa).

  • ポジティブ・コントロール
    • IHC-P: Human kidney tissue.
  • 特記事項


    Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.02% Sodium azide
    Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride
  • 精製度
    Whole antiserum
  • 一次抗体 備考
    Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab8927 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/200 - 1/800.
WB 1/400. Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).

ターゲット情報

  • 機能
    Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity). Involved in bone remodeling and homeostasis. In collaboration with RELA/p65 enhances NFATc1 promoter activity and positively regulates RANKL-induced osteoclast differentiation. Positively regulates self-renewal of liver cancer cells (PubMed:25985737).
  • 組織特異性
    Expressed in the brain, heart, kidney, lung, skeletal muscle and liver. Ubiquitously expressed in the embryo.
  • 関連疾患
    Alagille syndrome 2
    Hajdu-Cheney syndrome
  • 配列類似性
    Belongs to the NOTCH family.
    Contains 6 ANK repeats.
    Contains 35 EGF-like domains.
    Contains 3 LNR (Lin/Notch) repeats.
  • 翻訳後修飾
    Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane.
    Hydroxylated by HIF1AN.
    Can be either O-glucosylated or O-xylosylated at Ser-613 by POGLUT1.
  • 細胞内局在
    Cell membrane and Nucleus. Cytoplasm. Following proteolytical processing NICD is translocated to the nucleus. Retained at the cytoplasm by C8orf4 (PubMed:25985737).
  • Information by UniProt
  • 参照データベース
  • 別名
    • AGS2 antibody
    • hN2 antibody
    • Motch B antibody
    • N2 antibody
    • N2ECD antibody
    • N2ICD antibody
    • neurogenic locus notch homolog protein 2 antibody
    • NOTC2_HUMAN antibody
    • Notch 2 antibody
    • Notch 2 intracellular domain antibody
    • Notch Drosophila homolog 2 antibody
    • Notch homolog 2 antibody
    • Notch homolog 2 Drosophila antibody
    • Notch2 antibody
    see all

Anti-Notch2 antibody 画像



  • Predicted band size : 110 kDa

    Western Blot analysis for Notch 2 expression performed on 100µg of total protein lysate obtained from human mesothelial SV40 cells transfected with a plasmid encoding a constituvely active Notch 2 (intra cellular Notch 2).

    Lanes1: 24 hours after transfection. 
    Lane 2: 48 hours after transfection.
    Lane 3: 72 hours after transfection.
    Lane 4: Control cells 24 hours after transfection.
    Lane 5: Control cells 48 hours after transfection.
    Lane 6: Control cells 72 hours after transfection.

    The band at about 110kD represents active Notch 2. This band is not seen in the control cells, confirming that it is Notch 2. The intracellular domain of Notch 2 has a predicted band size of 110kD, corresponding to this band.

    Protein cell lysates were run on a 10% SDS-page gel, blotted onto Hybond C membrane, blocked overnight in PBS-Tween 20 supplemented with 5% Non-fat Milk and hybridized with ab8927 rabbit polyclonal a

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Notch2 with ab8927. Hematoxylin was used as the nuclear counterstain.

Anti-Notch2 antibody (ab8927) 使用論文

This product has been referenced in:
  • Krepler C  et al. Targeting Notch enhances the efficacy of ERK inhibitors in BRAF-V600E melanoma. Oncotarget 7:71211-71222 (2016). WB ; Human . Read more (PubMed: 27655717) »
  • Meng X  et al. GSI-I (Z-LLNle-CHO) inhibits ?-secretase and the proteosome to trigger cell death in precursor-B acute lymphoblastic leukemia. Leukemia 25:1135-46 (2011). Read more (PubMed: 21494254) »

See all 3 Publications for this product

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