Synthetic peptide corresponding to Human Notch2 aa 2396-2409 conjugated to Keyhole Limpet Haemocyanin (KLH) (Cysteine residue). Synthetic peptide:
NAAERTPSHSGHLQ
conjugated to KLH with a Cysteine residue linker, corresponding to amino acids 2396-2409 of Human Notch2 (NP_077719.2).NAAERTPSHSGHLQ
Our Abpromise guarantee covers the use of ab118824 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB | 1/400 - 1/2000. Predicted molecular weight: 265 kDa. | |
ELISA | 1/30000 - 1/90000. | |
IHC-P | 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
ICC/IF | Use at an assay dependent concentration. |
ab118824, at a 1/500 dilution, staining Notch2 in formalin fixed, paraffin embedded Human kidney tissue followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"