Anti-Notch1 intracellular domain 抗体 (ab83232)


  • 製品名Anti-Notch1 intracellular domain antibody
    Notch1 intracellular domain 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Notch1 intracellular domain
  • アプリケーション適用あり: WB, IHC-Frmore details
  • 種交差性
    交差種: Human, Apteronotus leptorhynchus
    交差が予測される動物種: Rat, Horse, Chicken, Guinea pig, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within c-terminal amino acids 2506-2555 (EHPFLTPSPE SPDQWSSSSP HSNVSDWSEG VSSPPTSMQS QIARIPEAFK) of Human Notch1 intracellular domain (NP_060087).

  • ポジティブ・コントロール
    • 293T cell lysate.



Our Abpromise guarantee covers the use of ab83232 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 273 kDa for Notch1 full length protein and 88 kDa for Notch1 intracellular domain. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-Fr Use at an assay dependent concentration.


  • 機能Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May be important for normal lymphocyte function. In altered form, may contribute to transformation or progression in some T-cell neoplasms. Involved in the maturation of both CD4+ and CD8+ cells in the thymus. May be important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, may function as a receptor for neuronal DNER and may be involved in the differentiation of Bergmann glia.
  • 組織特異性In fetal tissues most abundant in spleen, brain stem and lung. Also present in most adult tissues where it is found mainly in lymphoid tissues.
  • 関連疾患Defects in NOTCH1 are a cause of bicuspid aortic valve (BAV) [MIM:109730]. A common defect in the aortic valve in which two rather than three leaflets are present. It is often associated with aortic valve calcification and insufficiency. In extreme cases, the blood flow may be so restricted that the left ventricle fails to grow, resulting in hypoplastic left heart syndrome.
  • 配列類似性Belongs to the NOTCH family.
    Contains 5 ANK repeats.
    Contains 36 EGF-like domains.
    Contains 3 LNR (Lin/Notch) repeats.
  • 翻訳後修飾Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane.
    O-glycosylated on the EGF-like domains. Contains both O-linked fucose and O-linked glucose.
    Ubiquitinated; undergoes 'Lys-29'-linked polyubiquitination catalyzed by ITCH.
  • 細胞内局在Cell membrane and Nucleus. Following proteolytical processing NICD is translocated to the nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • hN1 antibody
    • Neurogenic locus Notch homolog protein 1 antibody
    • NICD antibody
    • NOTC1_HUMAN antibody
    • Notch 1 antibody
    • Notch 1 intracellular domain antibody
    • Notch homolog 1 translocation associated antibody
    • notch1 antibody
    • TAN1 antibody
    • Translocation associated Notch protein TAN1 antibody
    • Translocation-associated notch protein TAN-1 antibody
    see all

Anti-Notch1 intracellular domain antibody 画像

  • Anti-Notch1 intracellular domain antibody (ab83232) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Observed band size : 88 kDa (why is the actual band size different from the predicted?)
  • ab83232 staining Notch1 intracellular domain in 16µm sections of Apteronotus leptorhynchus brain tissue by Immunohistochemistry (Frozen sections).
    Tissue was fixed in paraformaldehyde, permeabilized using 0.3% Triton X-100, then blocked with 3% sheep serum, 1% BSA, 1% teleostean gelatine in TBS for 1 hour at 24°C, and then incubated with ab83232 at a 1/100 dilution for 18 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-rabbit polyclonal, used at a 1/200 dilution.

    See Abreview

Anti-Notch1 intracellular domain antibody (ab83232) 使用論文

ab83232 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Tongue epithelium)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate buffer followed by Trypsin
Permeabilization No
Specification Tongue epithelium
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative Paraformaldehyde

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Verified customer

投稿 Jun 13 2016

Application Western blot
Sample Rat Cell lysate - whole cell (neonatal rat primary cardiomyocytes)
Gel Running Conditions Reduced Denaturing
Loading amount 25 µg
Treatment 40nm Triptolide for 24hrs
Specification neonatal rat primary cardiomyocytes
Blocking step Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 15°C

Verified customer

投稿 Oct 30 2015

Application IHC - Wholemount
Sample Zebrafish Embryo (48hpf whole embryos)
Specification 48hpf whole embryos

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Verified customer

投稿 Feb 09 2015

Application Western blot
Sample Apteronotus leptorhynchus Tissue lysate - whole (Brain)
Loading amount 50 µg
Specification Brain
Gel Running Conditions Reduced Denaturing (4-15%)
Blocking step Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

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Verified customer

投稿 Sep 28 2012

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Application Immunohistochemistry (Frozen sections)
Sample Apteronotus leptorhynchus Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Permeabilization Yes - 0.3% Triton X-100
Blocking step 3% sheep serum, 1% BSA, 1% teleostean gelatine in TBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C

Dr. Ruxandra Sirbulescu

Verified customer

投稿 Nov 28 2011