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Our Abpromise guarantee covers the use of ab83232 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 273 kDa for Notch1 full length protein and 88 kDa for Notch1 intracellular domain. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-Fr||Use at an assay dependent concentration.|
Formalin-fixed, paraffin-embedded human lung tissue stained for Notch1 intracellular domain using ab83232 at 1/100 dilution (red) in immunohistochemical analysis, followed by Donkey anti-Rabbit-Cy3 at 1/200 dilution. DAPI (blue).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"