製品の概要

  • 製品名Anti-Notch1 antibody [EP1238Y]
    Notch1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1238Y] to Notch1
  • 特異性80% identities with Notch 2 and 81% with Notch 3
  • アプリケーション適用あり: IHC-P, Flow Cyt, WB, ICC/IFmore details
    適用なし: IP
  • 種交差性
    交差種: Mouse, Cow, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Notch1 aa 2500-2600.

  • ポジティブ・コントロール
    • WB: HEk293 cell lysate. IHC-P: Human brain tissue. ICC/IF: HeLa cells. Flow Cyt: HeLa cells.
  • 特記事項

    A trial size is available to purchase for this antibody.

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab52627 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/100 - 1/150. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

Flow Cyt 1/130 - 1/200.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB 1/1000 - 1/2000. Detects a band of approximately 125 kDa.

 

ICC/IF 1/100 - 1/150.

 

  • 追加情報Is unsuitable for IP.
  • ターゲット情報

    • 機能Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May be important for normal lymphocyte function. In altered form, may contribute to transformation or progression in some T-cell neoplasms. Involved in the maturation of both CD4+ and CD8+ cells in the thymus. May be important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, may function as a receptor for neuronal DNER and may be involved in the differentiation of Bergmann glia.
    • 組織特異性In fetal tissues most abundant in spleen, brain stem and lung. Also present in most adult tissues where it is found mainly in lymphoid tissues.
    • 関連疾患Defects in NOTCH1 are a cause of bicuspid aortic valve (BAV) [MIM:109730]. A common defect in the aortic valve in which two rather than three leaflets are present. It is often associated with aortic valve calcification and insufficiency. In extreme cases, the blood flow may be so restricted that the left ventricle fails to grow, resulting in hypoplastic left heart syndrome.
    • 配列類似性Belongs to the NOTCH family.
      Contains 5 ANK repeats.
      Contains 36 EGF-like domains.
      Contains 3 LNR (Lin/Notch) repeats.
    • 翻訳後修飾Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane.
      Phosphorylated.
      O-glycosylated on the EGF-like domains. Contains both O-linked fucose and O-linked glucose.
      Ubiquitinated; undergoes 'Lys-29'-linked polyubiquitination catalyzed by ITCH.
    • 細胞内局在Cell membrane and Nucleus. Following proteolytical processing NICD is translocated to the nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 9930111A19Rik antibody
      • AOS5 antibody
      • AOVD1 antibody
      • hN1 antibody
      • Lin-12 antibody
      • LIN12 antibody
      • Mis6 antibody
      • Motch A antibody
      • mT14 antibody
      • Neurogenic locus notch homolog protein 1 antibody
      • Neurogenic locus notch protein homolog antibody
      • NICD antibody
      • NOTC1_HUMAN antibody
      • Notch 1 antibody
      • Notch 1 intracellular domain antibody
      • NOTCH antibody
      • Notch gene homolog 1 (Drosophila) antibody
      • Notch homolog 1, translocation-associated (Drosophila) antibody
      • NOTCH, Drosophila, homolog of, 1 antibody
      • notch1 antibody
      • TAN1 antibody
      • Translocation associated notch homolog antibody
      • Translocation associated notch protein TAN 1 antibody
      • Translocation-associated notch protein TAN-1 antibody
      • xotch antibody
      see all

    Anti-Notch1 antibody [EP1238Y] 画像

    • Anti-Notch1 antibody [EP1238Y] (ab52627) at 1/2000 dilution (Purified) + HEK293 at 10 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Observed band size : 125 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

       

    • Immunohistochemical staining of paraffin-embedded human brain with purified ab52627 at a dilution of 1/150. A prediluted HRP polymer for rabbit IgG was used as the secondary and the sample was stained with hematoxylin. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

    • Immunofluorescent staining of HeLa cells fixed with 4% PFA using purified ab52627 at a dilution of 1/150. An Alexa Fluor® 555 goat anti-rabbit was used as the secondary and the sample was stained with DAPI. An Alexa Fluor® 555 goat anti-mouse was used at a dilution of 1/500 after ab52627 as the negative control and is shown in the bottom right hand panel.

    • Flow cytometry analysis of permeabilized HeLa cells (2% PFA, pink) with purified ab52627 at a 1/200 dilution, or negative control rabbit monoclonal IgG (green). The secondary antibody was FITC goat anti-rabbit.

    • Anti-Notch1 antibody [EP1238Y] (ab52627) at 1/2000 dilution (Purified) + HEK293 cell lysate at 10 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Observed band size : 125 kDa (why is the actual band size different from the predicted?)

      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Immunohistochemical staining of paraffin-embedded human brain with unpurified ab52627 at a dilution of 1/100. A prediluted HRP polymer for rabbit IgG was used as the secondary and the sample was stained with hematoxylin. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

    • Immunofluorescent staining of HeLa cells fixed with 4% PFA using unpurified ab52627 at a dilution of 1/100. An Alexa Fluor® 555 goat anti-rabbit was used as the secondary and the sample was stained with DAPI. An Alexa Fluor® 555 goat anti-mouse was used at a dilution of 1/500 as the negative control and is shown in the bottom right hand panel.

    Anti-Notch1 antibody [EP1238Y] (ab52627) 使用論文

    This product has been referenced in:
    • Peng C  et al. Norcantharidin Suppresses Colon Cancer Cell Epithelial-Mesenchymal Transition by Inhibiting the avß6-ERK-Ets1 Signaling Pathway. Sci Rep 6:20500 (2016). Read more (PubMed: 26846153) »
    • Chen X  et al. Suppression of HSP27 increases the anti-tumor effects of quercetin in human leukemia U937 cells. Mol Med Rep 13:689-96 (2016). WB ; Human . Read more (PubMed: 26648539) »

    See all 25 Publications for this product

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (CT26-WT tumor)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer pH6
    Permeabilization No
    Specification CT26-WT tumor
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 Jun 07 2016

    Application Western blot
    Sample Mouse Tissue lysate - other (brain)
    Gel Running Conditions Non-reduced Denaturing (10% acrylamide)
    Loading amount 25 µg
    Treatment 25uM DAPT
    Specification brain
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Ms. Victoria Lladó

    Verified customer

    投稿 Oct 16 2015

    Application Western blot
    Sample Human Cell lysate - whole cell (Airway Epithelial cells)
    Gel Running Conditions Reduced Denaturing (4-15%)
    Loading amount 30 µg
    Specification Airway Epithelial cells
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Username

    Abcam user community

    Verified customer

    投稿 Aug 26 2015

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (Embryonic kidney)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris pH=9
    Permeabilization No
    Specification Embryonic kidney
    Fixative Formaldehyde
    Username

    Abcam user community

    Verified customer

    投稿 Jul 30 2015

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris-EDTA, pH 9.0
    Sample Mouse Tissue sections (Testis)
    Specification Testis
    Permeabilization Yes - Triton
    Fixative Formaldehyde
    Username

    Dr. Daniel Murta

    Verified customer

    投稿 Jan 23 2014

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Loading amount 100 µg
    Gel Running Conditions Reduced Denaturing
    Sample Human Cell lysate - whole cell (ovarian cancer cell lines)
    Specification ovarian cancer cell lines
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    投稿 Aug 01 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Cow Tissue sections (tongue)
    Specification tongue
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer
    Permeabilization Yes - Triton
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
    Username

    Dr. Daniel Murta

    Verified customer

    投稿 May 15 2013

    Thank you for your reply.

    I can confirm that ab65297 and ab52627 both have immunogens that are also located within the NICD (amino acid 1754 to 2555).

    Therefore they also bind the region you are interested in. They areas specific as...

    Read More

    Thank you for your reply.

    I have created a new order free of charge for one vial of ab52627 (Anti-Notch1 antibody [EP1238Y]). The order number is xxxxx. You should receive an automatic confirmation.

    To check the status of the order ...

    Read More

    Thank you for contacting us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody’s batch.

    I appreciate you sent the images to illustrate the lack of staining you mention. Could you please confirm...

    Read More

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