製品の概要

  • 製品名Anti-Notch1 antibody - ChIP Grade
    Notch1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Notch1 - ChIP Grade
  • アプリケーション適用あり: WB, IHC-FoFr, ChIP, ICC/IF, IHC-Fr, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    Synthetic peptide (

    AASGHLGRSFLSGEPSQADV

    ) from C-terminal of human notch-1.

  • ポジティブ・コントロール
    • Breast carcinoma, ovarian carcinoma.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab27526 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. PubMed: 18156632
IHC-FoFr Use at an assay dependent concentration.
ChIP Use at an assay dependent concentration.
ICC/IF 1/100.
IHC-Fr Use at an assay dependent concentration.
IHC-P 1/200. for 10 minutes at room temperature. Staining of Formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 minutes followed by cooling at room temperature for 20 minutes.

ターゲット情報

  • 機能Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May be important for normal lymphocyte function. In altered form, may contribute to transformation or progression in some T-cell neoplasms. Involved in the maturation of both CD4+ and CD8+ cells in the thymus. May be important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, may function as a receptor for neuronal DNER and may be involved in the differentiation of Bergmann glia.
  • 組織特異性In fetal tissues most abundant in spleen, brain stem and lung. Also present in most adult tissues where it is found mainly in lymphoid tissues.
  • 関連疾患Defects in NOTCH1 are a cause of bicuspid aortic valve (BAV) [MIM:109730]. A common defect in the aortic valve in which two rather than three leaflets are present. It is often associated with aortic valve calcification and insufficiency. In extreme cases, the blood flow may be so restricted that the left ventricle fails to grow, resulting in hypoplastic left heart syndrome.
  • 配列類似性Belongs to the NOTCH family.
    Contains 5 ANK repeats.
    Contains 36 EGF-like domains.
    Contains 3 LNR (Lin/Notch) repeats.
  • 翻訳後修飾Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane.
    Phosphorylated.
    O-glycosylated on the EGF-like domains. Contains both O-linked fucose and O-linked glucose.
    Ubiquitinated; undergoes 'Lys-29'-linked polyubiquitination catalyzed by ITCH.
  • 細胞内局在Cell membrane and Nucleus. Following proteolytical processing NICD is translocated to the nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 9930111A19Rik antibody
    • AOS5 antibody
    • AOVD1 antibody
    • hN1 antibody
    • Lin-12 antibody
    • LIN12 antibody
    • Mis6 antibody
    • Motch A antibody
    • mT14 antibody
    • Neurogenic locus notch homolog protein 1 antibody
    • Neurogenic locus notch protein homolog antibody
    • NICD antibody
    • NOTC1_HUMAN antibody
    • Notch 1 antibody
    • Notch 1 intracellular domain antibody
    • NOTCH antibody
    • Notch gene homolog 1 (Drosophila) antibody
    • Notch homolog 1, translocation-associated (Drosophila) antibody
    • NOTCH, Drosophila, homolog of, 1 antibody
    • notch1 antibody
    • TAN1 antibody
    • Translocation associated notch homolog antibody
    • Translocation associated notch protein TAN 1 antibody
    • Translocation-associated notch protein TAN-1 antibody
    • xotch antibody
    see all

Anti-Notch1 antibody - ChIP Grade 画像

  • ab27526 staining Notch 1 in Mouse pancreatic cancer primary cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with acetone/methanol (1:1) and blocked with 10% serum for 1 hour at room temperature. Samples were incubated with primary antibody (1/100 in PBS) for 4 hours. An Alexa Fluor®488-conjugated Donkey anti-rabbit IgG polyclonal (ab150073) (1/1000) was used as the secondary antibody. Nuclei were counterstained with DAPI.

    See Abreview

  • Anti-Notch1 antibody - ChIP Grade (ab27526) at 1/500 dilution (in TBST for 8 hours at 4°C) + Whole cell lysate of human MiaPaCa cells at 50 µg

    Secondary
    An HRP-conjugated Donkey anti-rabbit IgG polyclonal at 1/5000 dilution
    Developed using the ECL technique

    Performed under non-reducing conditions.

    Observed band size : 120,250 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 10 minutes

    This image is courtesy of an anonymous Abreview

    Blocking Step: 5% Milk for 1 hour at room temperature.

    See Abreview

  • Staining of Ovarian carcinoma using ab27526
  • ab27526 staining Notch1 in mouse neural tube by immunohistochemistry (Frozen). Sections were paraformaldhyde fixed prior to blocking in 20% serum for 16 hours at 25°C and then incubated with ab27526 for 16 hours at 25°C. A Cy3 conjugated donkey polyclonal to rabbit Ig was used as the secondary antibody. Blue staining is nuclear staining with DAPI and the specific Notch signal is red.

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  • ab27526 staining Notch 1 in Human pancreatic neoplastic lesion tissue sections by Immunohistochemistry (IHC-P - formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at room temperature; antigen retrieval was by heat mediation in antigen unmasking solution. Samples were incubated with primary antibody (1/100 in PBS) for 8 hours at 4°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (ab6720) (1/1000) was used as the secondary antibody.

    See Abreview

  • ab27526 staining Notch1 in Mouse brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde and blocked with 3% serum for 30 minutes at 25°C. The sample was incubated with primary antibody (1/50 in 3% Horse serum - ab7484) at 4°C for 15 hours. An undiluted HRP-conjugated Horse anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

Anti-Notch1 antibody - ChIP Grade (ab27526) 使用論文

This product has been referenced in:
  • Li W  et al. Genome-wide analyses identify KLF4 as an important negative regulator in T-cell acute lymphoblastic leukemia through directly inhibiting T-cell associated genes. Mol Cancer 14:26 (2015). Read more (PubMed: 25644173) »
  • Nakajima K  et al. Galectin-3 inhibits osteoblast differentiation through notch signaling. Neoplasia 16:939-49 (2014). Human . Read more (PubMed: 25425968) »

See all 25 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Antigen retrieval step None
Sample Mouse Tissue sections (Brain)
Specification Brain
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Sep 27 2013


Unfortunately it is not possible for us to sell the pre-immune serum of our rabbits.
I am sorry that on this occasion I could not be of more help.

The concentration of lot # GR64051-2 of AB27526 Rabbit polyclonal to Notch1 - ChIP Grade is 0.200mg/ml.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Permeabilization Yes - Triton X-100 0.5%
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Dec 28 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Dec 19 2012

Thank you for contacting us.

Please follow the link below, to see many stainings of breast cancer (human though) from the human protein atlas project: http://www.proteinatlas.org/ENSG00000148400/cancer/breast+cancer

I hope this in...

Read More

Thank your for your inquiry.

According to the Swissprot homepage (http://www.uniprot.org/uniprot/P46531) the intracellular domain is from amino acid 1754 to 2555.

I can confirm after a quick alignment, that the immunogen sequence of...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Brain)
Specification Brain
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization Yes - 0.025% Triton X-100
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Apr 02 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Brain)
Specification Brain
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization Yes - 0.025% Triton X-100
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Apr 02 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (skin)
Specification skin
Fixative Paraformaldehyde
Antigen retrieval step Enzymatic - Buffer/Enzyme Used: proteinase K
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Jan 11 2012

1-10 of 26 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"