製品の概要

  • 製品名
    Anti-Notch1 antibody [A6]
    Notch1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [A6] to Notch1
  • 特異性
    This antibody is specific for Notch1.
  • アプリケーション
    適用あり: ICC/IF, Flow Cyt, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Recombinant full length protein corresponding to Human Notch1.

  • エピトープ
    Ligand binding region
  • ポジティブ・コントロール
    • Pancreas

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab44986 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
Flow Cyt Use at an assay dependent concentration.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

IHC-P 1/50.

ターゲット情報

  • 機能
    Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs. May be important for normal lymphocyte function. In altered form, may contribute to transformation or progression in some T-cell neoplasms. Involved in the maturation of both CD4+ and CD8+ cells in the thymus. May be important for follicular differentiation and possibly cell fate selection within the follicle. During cerebellar development, may function as a receptor for neuronal DNER and may be involved in the differentiation of Bergmann glia.
  • 組織特異性
    In fetal tissues most abundant in spleen, brain stem and lung. Also present in most adult tissues where it is found mainly in lymphoid tissues.
  • 関連疾患
    Defects in NOTCH1 are a cause of bicuspid aortic valve (BAV) [MIM:109730]. A common defect in the aortic valve in which two rather than three leaflets are present. It is often associated with aortic valve calcification and insufficiency. In extreme cases, the blood flow may be so restricted that the left ventricle fails to grow, resulting in hypoplastic left heart syndrome.
  • 配列類似性
    Belongs to the NOTCH family.
    Contains 5 ANK repeats.
    Contains 36 EGF-like domains.
    Contains 3 LNR (Lin/Notch) repeats.
  • 翻訳後修飾
    Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane.
    Phosphorylated.
    O-glycosylated on the EGF-like domains. Contains both O-linked fucose and O-linked glucose.
    Ubiquitinated; undergoes 'Lys-29'-linked polyubiquitination catalyzed by ITCH.
  • 細胞内局在
    Cell membrane and Nucleus. Following proteolytical processing NICD is translocated to the nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 9930111A19Rik antibody
    • AOS5 antibody
    • AOVD1 antibody
    • hN1 antibody
    • Lin-12 antibody
    • LIN12 antibody
    • Mis6 antibody
    • Motch A antibody
    • mT14 antibody
    • Neurogenic locus notch homolog protein 1 antibody
    • Neurogenic locus notch protein homolog antibody
    • NICD antibody
    • NOTC1_HUMAN antibody
    • Notch 1 antibody
    • Notch 1 intracellular domain antibody
    • NOTCH antibody
    • Notch gene homolog 1 (Drosophila) antibody
    • Notch homolog 1, translocation-associated (Drosophila) antibody
    • NOTCH, Drosophila, homolog of, 1 antibody
    • notch1 antibody
    • TAN1 antibody
    • Translocation associated notch homolog antibody
    • Translocation associated notch protein TAN 1 antibody
    • Translocation-associated notch protein TAN-1 antibody
    • xotch antibody
    see all

画像

  • ICC/IF image of ab44986 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab44986, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

参考文献

This product has been referenced in:
  • Wu X  et al. Autophagy regulates Notch degradation and modulates stem cell development and neurogenesis. Nat Commun 7:10533 (2016). IHC (PFA fixed), ICC/IF ; Mouse, Human . Read more (PubMed: 26837467) »
  • Cao Y  et al. Quantitative DNA hypomethylation of ligand Jagged1 and receptor Notch1 signifies occurrence and progression of breast carcinoma. Am J Cancer Res 5:1897-910 (2015). Read more (PubMed: 26269752) »

See all 11 Publications for this product

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