製品の概要

  • 製品名Anti-NOR1 antibody
    NOR1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to NOR1
  • アプリケーション適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Mouse
    交差が予測される動物種: Rat, Human, Pig
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human NOR1.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in NIH3T3 whole cell lysate. FFPE mouse brain tissue sections.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab94507 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).

ターゲット情報

  • 機能Binds to the B1A response-element.
  • 組織特異性Isoform alpha is highly expressed in skeletal muscle. Isoform beta is highly expressed in skeletal muscle and low expressed in fetal brain and placenta.
  • 関連疾患Defects in NR4A3 are a cause of Ewing sarcoma (ES) [MIM:612219]. A highly malignant, metastatic, primitive small round cell tumor of bone and soft tissue that affects children and adolescents. It belongs to the Ewing sarcoma family of tumors, a group of morphologically heterogeneous neoplasms that share the same cytogenetic features. They are considered neural tumors derived from cells of the neural crest. Ewing sarcoma represents the less differentiated form of the tumors. Note=A chromosomal aberration involving NR4A3 is found in patients with Erwing sarcoma. Translocation t(9;22)(q22-31;q11-12) with EWSR1.
    Note=A chromosomal aberration involving NR4A3 is a cause of a form of extraskeletal myxoid chondrosarcomas (EMC). Translocation t(9;17)(q22;q11) with TAF2N.
  • 配列類似性Belongs to the nuclear hormone receptor family. NR4 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CHN antibody
    • Chondrosarcoma, extraskeletal myxoid, fused to EWS antibody
    • CSMF antibody
    • MINOR antibody
    • Mitogen induced nuclear orphan receptor antibody
    • Mitogen-induced nuclear orphan receptor antibody
    • Neuron derived orphan receptor 1 antibody
    • Neuron derived orphan receptor antibody
    • Neuron-derived orphan receptor 1 antibody
    • NOR1 antibody
    • Nr4a3 antibody
    • NR4A3_HUMAN antibody
    • Nuclear hormone receptor NOR-1 antibody
    • Nuclear hormone receptor NOR1 antibody
    • Nuclear receptor subfamily 4 group A member 3 antibody
    • TEC antibody
    • Translocated in extraskeletal chondrosarcoma antibody
    see all

Anti-NOR1 antibody 画像

  • Anti-NOR1 antibody (ab94507) at 1 µg/ml + NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 68 kDa
    Observed band size : 68 kDa


    Exposure time : 20 minutes
  • IHC image of NOR1 staining in mouse brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab84507, 1µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-NOR1 antibody (ab94507) 使用論文

ab94507 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"