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This was raised against a yeast nucleolar prep, and screened by immunocytochemistry and western blotting.
Our Abpromise guarantee covers the use of ab4639 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000. Detects a band of approximately 67 kDa (predicted molecular weight: 57 kDa).|
|ICC/IF||Use at an assay dependent dilution.|
|IP||Use at an assay dependent dilution. Does not immunoprecipitate as well as another Nop5p monoclonal antibody, ab4640.|
ab4639 has not yet been referenced specifically in any publications.