製品の概要

  • 製品名Anti-Non Neuronal Enolase antibody
    Non Neuronal Enolase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Non Neuronal Enolase
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Chicken, Cow
  • 免疫原

    Synthetic peptide: GLCTGQIKTG APCRSERLAK YNQLLRIEEE LGSKAKFAGR NFRNPLAK, corresponding to a region within C terminal amino acids 387-434 of Human Non Neuronal Enolase

  • ポジティブ・コントロール
    • Jurkat cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab49191 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay 1/62500.

ターゲット情報

  • 機能Multifunctional enzyme that, as well as its role in glycolysis, plays a part in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.
    MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.
  • 組織特異性The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
  • パスウェイCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 4/5.
  • 配列類似性Belongs to the enolase family.
  • 発生段階During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells.
  • 翻訳後修飾ISGylated.
  • 細胞内局在Nucleus and Cytoplasm. Cell membrane. Cytoplasm > myofibril > sarcomere > M line. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 2-phospho-D-glycerate hydro-lyase antibody
    • alpha enolase like 1 antibody
    • Alpha-enolase antibody
    • bh3 interacting domain death agonist antibody
    • c-myc promoter-binding protein 1 antibody
    • C-myc promoter-binding protein antibody
    • c-myc promoter-binding protein-1 antibody
    • eno1 antibody
    • ENO1L1 antibody
    • ENOA_HUMAN antibody
    • enolase 1 (alpha) antibody
    • Enolase 1 antibody
    • HAP 47 antibody
    • HAP47 antibody
    • MBP-1 antibody
    • MPB 1 antibody
    • MPB-1 antibody
    • MPB1 antibody
    • MYC promoter-binding protein 1 antibody
    • NNE antibody
    • Non-neural enolase antibody
    • OTTHUMP00000001706 antibody
    • Phosphopyruvate hydratase antibody
    • Plasminogen-binding protein antibody
    • PPH antibody
    • tau-crystallin antibody
    see all

Anti-Non Neuronal Enolase antibody 画像

  • Anti-Non Neuronal Enolase antibody (ab49191) at 1.25 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution
    Developed using the ECL technique

    Predicted band size : 47 kDa
    Observed band size : 47 kDa

Anti-Non Neuronal Enolase antibody (ab49191) 使用論文

This product has been referenced in:
  • Chenau J  et al. The cell line secretome, a suitable tool for investigating proteins released in vivo by tumors: application to the study of p53-modulated proteins secreted in lung cancer cells. J Proteome Res 8:4579-91 (2009). WB ; Human . Read more (PubMed: 19639960) »

See 1 Publication for this product

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