Anti-non-muscle Myosin IIB 抗体 (ab83984)

製品の概要

  • 製品名Anti-non-muscle Myosin IIB antibody
    non-muscle Myosin IIB 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to non-muscle Myosin IIB
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 540 - 589 (WFPKATDKTF VEKLVQEQGS HSKFQKPRQL KDKADFCIIH YAGKVDYKAD) of Human non-muscle Myosin IIB (NP_005955).

  • ポジティブ・コントロール
    • Human fetal lung lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab83984 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 229 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能Cellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
  • 組織特異性Isoform 1 is expressed in cerebellum and spinal chord. Isoform 2 is expressed in cerebrum and retina. Isoform 3 is expressed in the cerebrum and to a much lower extent in cerebellum.
  • 配列類似性Contains 1 IQ domain.
    Contains 1 myosin head-like domain.
  • ドメインThe rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
  • 翻訳後修飾Phosphorylated by ABL2.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cellular myosin heavy chain antibody
    • Cellular myosin heavy chain type B antibody
    • Cellular myosin heavy chain type B type B antibody
    • MGC134913 antibody
    • MGC134914 antibody
    • MYH 10 antibody
    • Myh10 antibody
    • MYH10_HUMAN antibody
    • Myosin 10 antibody
    • Myosin heavy chain 10 antibody
    • Myosin heavy chain 10 non muscle antibody
    • Myosin heavy chain antibody
    • Myosin heavy chain non muscle 11b antibody
    • Myosin heavy chain nonmuscle IIb antibody
    • Myosin heavy chain nonmuscle type B antibody
    • Myosin heavy polypeptide 10 non muscle antibody
    • Myosin-10 antibody
    • Myosin10 antibody
    • NMMHC B antibody
    • NMMHC II b antibody
    • NMMHC II-b antibody
    • NMMHC IIB antibody
    • NMMHC-B antibody
    • NMMHC-IIB antibody
    • NMMHCB antibody
    • Non muscle myosin heavy chain B antibody
    • Non muscle myosin heavy chain IIB antibody
    • Non muscle myosin II heavy chain B antibody
    • non-muscle IIb antibody
    • Non-muscle myosin heavy chain B antibody
    • Non-muscle myosin heavy chain IIb antibody
    • Nonmuscle myosin heavy chain B antibody
    • Nonmuscle myosin heavy chain IIB antibody
    • Nonmuscle myosin II heavy chain B antibody
    • Nonmuscle myosin IIB antibody
    • type B antibody
    see all

Anti-non-muscle Myosin IIB antibody 画像

  • Anti-non-muscle Myosin IIB antibody (ab83984) at 1 µg/ml (in 5% skim milk / PBS buffer) + human fetal lung lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 229 kDa
    Observed band size : >168 kDa (why is the actual band size different from the predicted?)

Anti-non-muscle Myosin IIB antibody (ab83984) 使用論文

ab83984 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing (10% gel)
Sample Human Cell lysate - whole cell (HUES7)
Specification HUES7
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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投稿 Apr 03 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"