1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Plays an essential role in the survival of diffuse-type gastric cancer cells. May be involved in regulation of gene expression at the post-transcriptional level or in ribosome biogenesis in cancer cells.
Expressed in various diffuse-type gastric cancers. Detected at lower levels in skeletal muscle.
Detection of human NOL8 by Western Blot of Immunoprecipitate.
ab95898 at 0.1µg/ml staining NOL8 in HeLa cell lysate immunoprecipitated using ab95898 at 3µg/mg (1mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.