製品の概要

  • 製品名
    Anti-NOL8 antibody
  • 製品の詳細
    Rabbit polyclonal to NOL8
  • アプリケーション
    適用あり: WB, IP, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residue 200 and 250 of human NOL8 (NP_060418.4).

  • ポジティブ・コントロール
    • HeLa, 293T cell lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate, pH 7-8
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab95898 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 132 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    Plays an essential role in the survival of diffuse-type gastric cancer cells. May be involved in regulation of gene expression at the post-transcriptional level or in ribosome biogenesis in cancer cells.
  • 組織特異性
    Expressed in various diffuse-type gastric cancers. Detected at lower levels in skeletal muscle.
  • 配列類似性
    Contains 1 RRM (RNA recognition motif) domain.
  • 翻訳後修飾
    Phosphorylated.
  • 細胞内局在
    Nucleus > nucleolus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C9orf34 antibody
    • NOL8 antibody
    • NOL8_HUMAN antibody
    • NOP132 antibody
    • Nucleolar protein 8 antibody
    • Nucleolar protein Nop132 antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling NOL8 with ab95898 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-NOL8 antibody (ab95898) at 0.1 µg/ml

    Lane 1 : HeLa cell lysate at 50 µg
    Lane 2 : HeLa cell lysate at 15 µg
    Lane 3 : HeLa cell lysate at 5 µg
    Lane 4 : 293T cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 132 kDa


    Exposure time : 30 seconds
  • Detection of human NOL8 by Western Blot of Immunoprecipitate. ab95898 at 0.1µg/ml staining NOL8 in HeLa cell lysate immunoprecipitated using ab95898 at 3µg/mg (1mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 10 seconds.

参考文献

ab95898 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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