製品の概要
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製品名Anti-NOL5A antibody
NOL5A 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to NOL5A
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由来種Rabbit
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アプリケーション適用あり: IHC-P, WBmore details
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種交差性交差種: Human
交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
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免疫原
Synthetic peptide corresponding to Human NOL5A aa 193-242 (internal sequence).
Sequence:YGYHFPELVKIINDNATYCRLAQFIGNRRELNEDKLEKLEELTMDGAKAK
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ポジティブ･コントロール
- 721_B cell lysate
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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バッファーPreservative: None
Constituents: 2% Sucrose, PBS -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab74783 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | Use a concentration of 5 µg/ml. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 66 kDa (predicted molecular weight: 66 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T. |
ターゲット情報
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関連性NOL5A is similar in sequence to Nop56p, a yeast nucleolar protein that is part of a complex with the nucleolar proteins Nop58p and fibrillarin. Like Nop56p, NOL5A is found in the nucleolus and is though to be required for 60S ribosomal subunit biogenesis. Multiple transcript variants encoding several isoforms have been identified for this gene, but the full-length nature of most of them is not yet determined.
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細胞内局在Nuclear; nucleolus
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参照データベース
- Entrez Gene: 10528 Human
- Entrez Gene: 67134 Mouse
- Entrez Gene: 362214 Rat
- Omim: 614154 Human
- SwissProt: O00567 Human
- SwissProt: Q9D6Z1 Mouse
- Unigene: 145204 Rat
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別名
- NOL5A antibody
- NOP56 antibody
- NOP56 ribonucleoprotein antibody
see all
画像
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Western blot - Anti-NOL5A antibody (ab74783)Anti-NOL5A antibody (ab74783) at 1 µg/ml + 721_B cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 66 kDa
Observed band size: 66 kDa
Gel concentration: 12% -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOL5A antibody (ab74783)ab74783 at 5 µg/ml staining NOL5A in Human placenta by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
参考文献
ab74783 has not yet been referenced specifically in any publications.
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"