製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to NOL12
  • アプリケーション
    適用あり: WB, IP, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Rhesus monkey
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 163-213 (SLTASLHAHS RKKVKRKHPR RAQDSKKPPR APRTSKAQRR RLTGKARHSG E) of Human NOL12 (NP_077289.1).

  • ポジティブ・コントロール
    • HeLa whole cell lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate buffer, pH 7-8
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG

アプリケーション

Our Abpromise guarantee covers the use of ab99276 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 25 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    May bind to 28S rRNA.
  • 配列類似性
    Belongs to the RRP17 family.
  • 細胞内局在
    Nucleus > nucleolus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C78541 antibody
    • dJ37E16.7 antibody
    • FLJ34609 antibody
    • MGC3731 antibody
    • Nol12 antibody
    • NOL12_HUMAN antibody
    • Nop25 antibody
    • Nucleolar protein 12 antibody
    • Nucleolar protein of 25 kDa antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling NOL12 with ab99276 at 1/1000 (1µg/mg). Detection: DAB.
  • All lanes : Anti-NOL12 antibody (ab99276) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg

    Developed using the ECL technique

    Predicted band size : 25 kDa


    Exposure time : 3 minutes
  • Detection of NOL12 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab99276 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.

参考文献

ab99276 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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