Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling NOL12 with ab99276 at 1/1000 (1µg/mg). Detection: DAB.
Western blot - NOL12 antibody (ab99276)
All lanes : Anti-NOL12 antibody (ab99276) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 25 kDa
Exposure time: 3 minutes
Immunoprecipitation - NOL12 antibody (ab99276)
Detection of NOL12 in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab99276 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 30 seconds.