製品の概要

  • 製品名Anti-Nogo Receptor antibody
    Nogo Receptor 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Nogo Receptor
  • アプリケーション適用あり: WB, IHC-Fr, IHC-Pmore details
  • 種交差性
    交差種: Rat, Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Rat Nogo Receptor.

    (Peptide available as ab27807.)

  • ポジティブ・コントロール
    • Rat Brain

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab26291 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).
IHC-Fr Use at an assay dependent concentration. PubMed: 17984503
IHC-P 1/400. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

ターゲット情報

Anti-Nogo Receptor antibody 画像

  • Anti-Nogo Receptor antibody (ab26291) at 1 µg/ml + Rat Brain Tissue Lysate (Whole Cell) at 20 µg

    Secondary
    Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 51 kDa
    Observed band size : 51 kDa
    Additional bands at : 49 kDa (possible truncated form).
  • (Image courtesy of Human Protein Atlas)
    ab26291 staining Nogo Receptor protein in neuronal cells of normal Human cerebral cortex. Paraffin embedded human cerebral cortex tissue was incubated with ab26291(1/400 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

Anti-Nogo Receptor antibody (ab26291) 使用論文

This product has been referenced in:
  • Fernandez-Enright F  et al. Novel implications of Lingo-1 and its signaling partners in schizophrenia. Transl Psychiatry 4:e348 (2014). WB ; Human . Read more (PubMed: 24448210) »
  • Cui Z  et al. Oncomodulin/Truncated protamine-mediated nogo-66 receptor small interference RNA delivery promotes axon regeneration in retinal ganglion cells. Mol Cells 37:613-9 (2014). Read more (PubMed: 25134537) »

See all 4 Publications for this product

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Antigen retrieval step None
Sample Rat Tissue sections (Motor cortex)
Specification Motor cortex
Permeabilization No
Fixative Paraformaldehyde
Username

Mr. David Pruitt

Verified customer

投稿 Jan 27 2014

Vielen Dank für Ihre Anfrage.

Da wir auf unserer Webseite keine expliziten Angaben unter der WB Applikation gemacht haben, können Sie Ihr Standardt-Protokoll verwenden, da wir nur Protokollempfehlungen bei bekannten Problemen (z.B....

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Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten.

Wie am Freitag besprochen, möchte ich Ihnen den ab26291 Anti-Nogo Receptor antibody aus unserem Sortiment empfehlen, da nicht nur Ihrer Test- Spezies "...

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Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Brain Cortex and Hippocampus)
Specification Brain Cortex and Hippocampus
Username

Dr. Sophie Pezet

Verified customer

投稿 Jul 29 2011




Es freut mich zu hören, dass Sie unser Angebot unseren Antikörper ab26291 in WB zu benutzen und dann eine Abbildung einzusenden, annehmen möchten. Wie am Telefon besprochen können Sie den Gutscheincode zum Kauf eines ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"