Anti-Nogo A+B 抗体 (ab47085)
Key features and details
- Rabbit polyclonal to Nogo A+B
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Rat, Human, African green monkey
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Nogo A+B antibody -
製品の詳細
Rabbit polyclonal to Nogo A+B -
由来種
Rabbit -
特異性
ab47085 recognises Nogo A and Nogo B2 and B1 -
アプリケーション
適用あり: IHC-P, ICC/IF, WBmore details -
種交差性
交差種: Mouse, Rat, Human, African green monkey -
免疫原
Synthetic peptide, sequence taken from within amino acids 1-50 of human Nogo B.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading...
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精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab47085の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P | (1) |
Use at an assay dependent concentration.
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ICC/IF | (5) |
1/200.
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WB | (4) |
1/500 - 1/2000. Detects a band of approximately >200 kDa.
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特記事項 |
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IHC-P
Use at an assay dependent concentration. |
ICC/IF
1/200. |
WB
1/500 - 1/2000. Detects a band of approximately >200 kDa. |
ターゲット情報
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関連性
NOGO is a potent neurite outgrowth inhibitor which may also help block the regeneration of the central nervous system in higher vertebrates. Adult mammalian axon regeneration is generally successful in the peripheral nervous system but poor in the central nervous system. Inhibition results from physical barriers imposed by glial scars, a lack of neurotrophic factors, and growth-inhibitory molecules associated with myelin, the insulating axon sheath. These molecules include NI35, myelin-associated glycoprotein (159460), and Nogo. Several isoforms (A-E) of NOGO exist. -
細胞内局在
Endoplasmic reticulum; endoplasmic reticulum membrane; multi-pass membrane protein. Note=Anchored to the membrane of the endoplasmic reticulum through 2 putative transmembrane domains. -
参照データベース
- Entrez Gene: 57142 Human
- Entrez Gene: 68585 Mouse
- Entrez Gene: 83765 Rat
- Omim: 604475 Human
- SwissProt: Q9NQC3 Human
- SwissProt: Q99P72 Mouse
- SwissProt: Q9JK11 Rat
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別名
- Nogo A antibody
- Nogo antibody
- Nogo B antibody
see all
画像
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All lanes : Anti-Nogo A+B antibody (ab47085) at 1/2000 dilution
Lane 1 : Human brain tissue lysate
Lane 2 : Mouse brain tissue lysate
Lane 3 : Rat brain tissue lysate
Observed band size: >200,41,43,48-50 kDa why is the actual band size different from the predicted? -
ab47085 staining Nogo A+B in African Green Monkey COS-7 cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 3% BSA for 1 hour at 23°C. Samples were incubated with primary antibody (1/500 in PBS-BSA) for 1 hour at 23°C. An Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.
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Immunocytochemistry stainning for Nogo A+B in Cor1 neural stem cells from mouse using Rabbit polyclonal to Nogo A+B (ab47085; 1/200 incubated for 2h at RT); Immunoreactivity was detected in cell-body as well as in neurites.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (18)
ab47085 は 18 報の論文で使用されています。
- Golchoubian B et al. Reticulon-like REEP4 at the inner nuclear membrane promotes nuclear pore complex formation. J Cell Biol 221:N/A (2022). PubMed: 34874453
- Chen K et al. Excitation spectral microscopy for highly multiplexed fluorescence imaging and quantitative biosensing. Light Sci Appl 10:97 (2021). PubMed: 33963178
- Parashar S et al. Endoplasmic reticulum tubules limit the size of misfolded protein condensates. Elife 10:N/A (2021). PubMed: 34467852
- Maimaris G et al. Regulation of ER Composition and Extent, and Putative Action in Protein Networks by ER/NE Protein TMEM147. Int J Mol Sci 22:N/A (2021). PubMed: 34638576
- Calizo RC et al. Cell shape regulates subcellular organelle location to control early Ca2+ signal dynamics in vascular smooth muscle cells. Sci Rep 10:17866 (2020). PubMed: 33082406