製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Nogo A
  • 特異性
    ab62024 is specific for Nogo A and Nogo E.
  • アプリケーション
    適用あり: ICC/IF, IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Raised against a 19 amino acid synthetic peptide from near the centre of Human Nogo A (GenBank accession no. NP_065393).

  • ポジティブ・コントロール
    • Human brain tissue and mouse brain tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab62024 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF
IHC-P
WB
  • 追加情報
    ICC/IF: Use at a concentration of 1 µg/ml.
    IHC-P: Use at a concentration of 2.5 µg/ml.
    WB: Use at a concentration of 0.5 - 1.0 µg/ml. Detects a band of approximately 180 kDa (predicted molecular weight: 130 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Developmental neurite growth regulatory factor with a role as a negative regulator of axon-axon adhesion and growth, and as a facilitator of neurite branching. Regulates neurite fasciculation, branching and extension in the developing nervous system. Involved in down-regulation of growth, stabilization of wiring and restriction of plasticity in the adult CNS. Regulates the radial migration of cortical neurons via an RTN4R-LINGO1 containing receptor complex (By similarity). Isoform 2 reduces the anti-apoptotic activity of Bcl-xl and Bcl-2. This is likely consecutive to their change in subcellular location, from the mitochondria to the endoplasmic reticulum, after binding and sequestration. Isoform 2 and isoform 3 inhibit BACE1 activity and amyloid precursor protein processing.
    • 組織特異性
      Isoform 1 is specifically expressed in brain and testis and weakly in heart and skeletal muscle. Isoform 2 is widely expressed except for the liver. Isoform 3 is expressed in brain, skeletal muscle and adipocytes. Isoform 4 is testis-specific.
    • 配列類似性
      Contains 1 reticulon domain.
    • ドメイン
      Three regions, residues 59-172, 544-725 and the loop 66 amino acids, between the two transmembrane domains, known as Nogo-66 loop, appear to be responsible for the inhibitory effect on neurite outgrowth and the spreading of neurons. This Nogo-66 loop, mediates also the binding of RTN4 to its receptor.
    • 細胞内局在
      Endoplasmic reticulum membrane. Anchored to the membrane of the endoplasmic reticulum through 2 putative transmembrane domains.
    • Information by UniProt
    • 参照データベース
    • 別名
      • ASY antibody
      • Foocen antibody
      • Human NogoA antibody
      • My043 protein antibody
      • Nbla00271 antibody
      • Nbla10545 antibody
      • neurite growth inhibitor 220 antibody
      • Neurite outgrowth inhibitor antibody
      • Neuroendocrine-specific protein antibody
      • Neuroendocrine-specific protein C homolog antibody
      • NI220/250 antibody
      • Nogo A antibody
      • NOGO antibody
      • Nogo protein antibody
      • NOGO-A antibody
      • Nogo-B antibody
      • Nogo-C antibody
      • NSP antibody
      • NSP-CL antibody
      • Reticulon 4 antibody
      • reticulon 5 antibody
      • Reticulon-4 antibody
      • Reticulon-5 antibody
      • RTN 4 antibody
      • RTN 4A antibody
      • RTN xL antibody
      • RTN-x antibody
      • Rtn4 antibody
      • RTN4-A antibody
      • RTN4-B1 antibody
      • RTN4-B2 antibody
      • RTN4-C antibody
      • RTN4_HUMAN antibody
      see all

    画像

    • Immunohistochemistry (Formalin-fixed paraffin embedded sections) of mouse brain tissue labeling Nogo A with Anti-Nogo A antibody (ab62024) at 5μg/ml.
    • Lane 1 : Anti-Nogo A antibody (ab62024) at 0.5 µg/ml
      Lane 2 : Anti-Nogo A antibody (ab62024) at 1 µg/ml

      Lane 1 : Human brain tissue lysate
      Lane 2 : Human brain tissue lysate

      Lysates/proteins at 15 µg per lane.

      Secondary
      anti-rabbit IgG

      Predicted band size : 130 kDa
      Observed band size : 180 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 115 kDa,155 kDa. We are unsure as to the identity of these extra bands.
    • ab62024, at 2.5µg/ml, staining Mouse Nogo A in brain tissue, by Immunohistochemistry
    • ICC/IF image of ab62024 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab62024, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Immunofluorescence of NogoA in Mouse Brain cells using ab62024 at 20 ug/ml.

    参考文献

    This product has been referenced in:
    • He R  et al. Effect of fasudil on cognitive function following status convulsion in rats. Mol Med Rep 16:119-126 (2017). WB ; Rat . Read more (PubMed: 28534935) »
    • Travaglia A  et al. Developmental changes in plasticity, synaptic, glia and connectivity protein levels in rat dorsal hippocampus. Neurobiol Learn Mem 135:125-138 (2016). Read more (PubMed: 27523749) »

    See all 3 Publications for this product

    レビューと Q&A

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa cell)
    Permeabilization
    Yes - 0.1-0.25% Triton X 100
    Specification
    HeLa cell
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Username

    Dr. Daniel Levy

    Verified customer

    投稿 May 23 2017

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa cell lysate)
    Gel Running Conditions
    Reduced Denaturing (5-20% gradient gel)
    Loading amount
    10 µg
    Treatment
    Benzonase nuclease for 5-10 min
    Specification
    HeLa cell lysate
    Blocking step
    LICOR Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0% · Temperature: RT°C
    Username

    Dr. Daniel Levy

    Verified customer

    投稿 May 23 2017

    Thank you for contacting us yesterday to discuss the comments your reviewer had made.

    I have had a look into your enquiry and have come up with the following information:

    Nogo A is a potent neurite outgrowth inhibitor and plays a ro...

    Read More

    Thank you for that additional information. This has helped clarify the issues that you have been experiencing.

    I am sorry that this antibody has not worked as it should have. The image that you have provided us shows definitively that this i...

    Read More

    Thank you for contacting Abcam.

    It appears as if you may have recognized an isoform of Nogo. The immunogen sequence used corresponds to aa117601192 of Nogo A. However, this sequence is also present in each of the four known isoforms of Nogo....

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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