Anti-Nodal 抗体 [EP2058Y] (ab81287)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2058Y] to Nodal
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-Nodal antibody [EP2058Y]
Nodal 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP2058Y] to Nodal -
由来種
Rabbit -
特異性
ab81287 detects Pro-Nodal and Nodal. This antibody is unable to detect signal in endogenous samples.
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アプリケーション
適用あり: WBmore details
適用なし: IHC-P or IP -
種交差性
交差種: Human
交差が予測される動物種: Mouse -
免疫原
Synthetic peptide within Human Nodal aa 300-400 (C terminal). The exact sequence is proprietary.
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ポジティブ・コントロール
- Recombinant nodal protein.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP2058Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab81287の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000. Predicted molecular weight: 39 kDa.
(Pro-Nodal) and 13 kDa (Nodal). |
特記事項 |
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WB
1/2000. Predicted molecular weight: 39 kDa. (Pro-Nodal) and 13 kDa (Nodal). |
ターゲット情報
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機能
Essential for mesoderm formation and axial patterning during embryonic development. -
関連疾患
Defects in NODAL are the cause of visceral heterotaxy autosomal type 5 (HTX5) [MIM:270100]. A form of visceral heterotaxy, a complex disorder due to disruption of the normal left-right asymmetry of the thoracoabdominal organs. It results in an abnormal arrangement of visceral organs, and a wide variety of congenital defects. Clinical features of visceral heterotaxy autosomal type 5 include situs inversus viscerum or situs ambiguus, congenital heart defect, transposition of the great vessels ventricular septal defect, atrial septal defect, truncuscommunis, and dextrocardia. -
配列類似性
Belongs to the TGF-beta family. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 4838 Human
- Entrez Gene: 18119 Mouse
- Omim: 601265 Human
- SwissProt: Q96S42 Human
- SwissProt: P43021 Mouse
- Unigene: 370414 Human
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別名
- HTX5 antibody
- MGC138230 antibody
- Nodal antibody
see all
画像
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Anti-Nodal antibody [EP2058Y] (ab81287) at 1/2000 dilution + Recombinant nodal protein at 10ng
Secondary
HRP conjugated goat anti-rabbit at 1/2000 dilution
Predicted band size: 39 kDa
Observed band size: 15 kDa why is the actual band size different from the predicted?
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab81287 は 3 報の論文で使用されています。
- Li H et al. Nodal induced by hypoxia exposure contributes to dacarbazine resistance and the maintenance of stemness in melanoma cancer stem-like cells. Oncol Rep 39:2855-2864 (2018). PubMed: 29693169
- Strizzi L et al. Effects of a novel Nodal-targeting monoclonal antibody in melanoma. Oncotarget 6:34071-86 (2015). Sandwich ELISA ; Human . PubMed: 26460952
- Malchenko S et al. Cancer hallmarks in induced pluripotent cells: new insights. J Cell Physiol 225:390-3 (2010). WB ; Human . PubMed: 20568225