製品の概要
- 製品名Anti-Nodal antibody
Nodal 一次抗体 製品一覧 - 製品の詳細Mouse monoclonal to Nodal
- アプリケーション適用あり: WB, IHC-P, ICC/IF, Flow Cytmore details
- 種交差性交差種: Human
- 免疫原
Recombinant fragment: RCEGECPNPV GEEFHPTNHA YIQSLLKRYQ PHRVPSTCCA PVKTKPLSML YVDNGRVLLD HHKDMIVEEC GC, corresponding to amino acids 275-347 of Human Nodal
製品の特性
- 製品の状態Liquid
- 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- バッファーPreservative: None
PBS, pH 7.2 -
Concentration information loading... - 精製度Protein G purified
- ポリ/モノモノクローナル
- アイソタイプIgG1
- 軽鎖の種類kappa
- 研究分野
関連製品
- Compatible Secondaries
- Isotype control
- Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab55676 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 40 kDa. | |
| IHC-P | Use a concentration of 3 µg/ml. | |
| ICC/IF | Use at an assay dependent concentration. | |
| Flow Cyt | Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ターゲット情報
- 機能Essential for mesoderm formation and axial patterning during embryonic development.
- 関連疾患Defects in NODAL are the cause of visceral heterotaxy autosomal type 5 (HTX5) [MIM:270100]. A form of visceral heterotaxy, a complex disorder due to disruption of the normal left-right asymmetry of the thoracoabdominal organs. It results in an abnormal arrangement of visceral organs, and a wide variety of congenital defects. Clinical features of visceral heterotaxy autosomal type 5 include situs inversus viscerum or situs ambiguus, congenital heart defect, transposition of the great vessels ventricular septal defect, atrial septal defect, truncuscommunis, and dextrocardia.
- 配列類似性Belongs to the TGF-beta family.
- 細胞内局在Secreted.
- Information by UniProt
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参照データベース
- Entrez Gene: 4838 Human
- Omim: 601265 Human
- SwissProt: Q96S42 Human
- Unigene: 370414 Human
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別名
- HTX5 antibody
- MGC138230 antibody
- Nodal antibody
see all
Anti-Nodal antibody 画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nodal antibody (ab55676)Nodal antibody (ab55676) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human endometrium cancer. -
Western blot - Nodal antibody (ab55676)
Predicted band size : 40 kDa
Nodal antibody (ab55676) at 1ug/lane + HeLa cell lysate at 25ug/lane. -
Immunocytochemistry/ Immunofluorescence - Nodal antibody (ab55676)ICC/IF image of ab55676 stained mouse embryonic stem cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55676, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Flow Cytometry - Nodal antibody (ab55676)Overlay histogram showing HEK293 cells stained with ab55676 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55676, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 100% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
Anti-Nodal antibody (ab55676) 使用論文
This product has been referenced in:
- Strizzi L et al. Translational significance of Nodal, Cripto-1 and Notch4 in adult nevi. Oncol Lett 12:1349-1354 (2016). IHC . Read more (PubMed: 27446436) »
- Chen J et al. Embryonic morphogen nodal is associated with progression and poor prognosis of hepatocellular carcinoma. PLoS One 9:e85840 (2014). IHC-P ; Human . Read more (PubMed: 24465741) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"