製品の概要
-
製品名Anti-Nodal antibody
Nodal 一次抗体 製品一覧 -
製品の詳細Mouse monoclonal to Nodal
-
由来種Mouse
-
アプリケーション適用あり: WB, IHC-P, ICC/IF, Flow Cytmore details
-
種交差性交差種: Human
-
免疫原
Recombinant fragment: RCEGECPNPV GEEFHPTNHA YIQSLLKRYQ PHRVPSTCCA PVKTKPLSML YVDNGRVLLD HHKDMIVEEC GC, corresponding to amino acids 275-347 of Human Nodal
-
特記事項
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.
製品の特性
-
製品の状態Liquid
-
保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
-
バッファーPreservative: None
PBS, pH 7.2 -
Concentration information loading... -
精製度Protein G purified
-
ポリ/モノモノクローナル
-
アイソタイプIgG1
-
軽鎖の種類kappa
-
研究分野
関連製品
-
Compatible Secondaries
-
Immunohistochemistry kits
-
Isotype control
-
Recombinant Protein
アプリケーション
Our Abpromise guarantee covers the use of ab55676 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 40 kDa. | |
| IHC-P | Use a concentration of 3 µg/ml. | |
| ICC/IF | Use at an assay dependent concentration. | |
| Flow Cyt | Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
ターゲット情報
-
機能Essential for mesoderm formation and axial patterning during embryonic development.
-
関連疾患Defects in NODAL are the cause of visceral heterotaxy autosomal type 5 (HTX5) [MIM:270100]. A form of visceral heterotaxy, a complex disorder due to disruption of the normal left-right asymmetry of the thoracoabdominal organs. It results in an abnormal arrangement of visceral organs, and a wide variety of congenital defects. Clinical features of visceral heterotaxy autosomal type 5 include situs inversus viscerum or situs ambiguus, congenital heart defect, transposition of the great vessels ventricular septal defect, atrial septal defect, truncuscommunis, and dextrocardia.
-
配列類似性Belongs to the TGF-beta family.
-
細胞内局在Secreted.
- Information by UniProt
-
参照データベース
- Entrez Gene: 4838 Human
- Omim: 601265 Human
- SwissProt: Q96S42 Human
- Unigene: 370414 Human
-
別名
- HTX5 antibody
- MGC138230 antibody
- Nodal antibody
see all
画像
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nodal antibody (ab55676)Nodal antibody (ab55676) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human endometrium cancer. -
Western blot - Nodal antibody (ab55676)Nodal antibody (ab55676) at 1ug/lane + HeLa cell lysate at 25ug/lane. -
Immunocytochemistry/ Immunofluorescence - Nodal antibody (ab55676)ICC/IF image of ab55676 stained mouse embryonic stem cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55676, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. -
Flow Cytometry - Nodal antibody (ab55676)Overlay histogram showing HEK293 cells stained with ab55676 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55676, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 100% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
プロトコール
参考文献
This product has been referenced in:
- Jiang Y et al. ALDH enzyme activity is regulated by Nodal and histamine in the A549 cell line. Oncol Lett 14:6955-6961 (2017). Read more (PubMed: 29181106) »
- Strizzi L et al. Translational significance of Nodal, Cripto-1 and Notch4 in adult nevi. Oncol Lett 12:1349-1354 (2016). IHC . Read more (PubMed: 27446436) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"