Anti-Nodal 抗体 [5C3] (ab55676)
Key features and details
- Mouse monoclonal [5C3] to Nodal
- Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
- Reacts with: Mouse, Human
- Isotype: IgG1
製品の概要
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製品名
Anti-Nodal antibody [5C3]
Nodal 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [5C3] to Nodal -
由来種
Mouse -
アプリケーション
適用あり: WB, IHC-P, ICC/IF, Flow Cytmore details -
種交差性
交差種: Mouse, Human -
免疫原
Recombinant fragment: RCEGECPNPV GEEFHPTNHA YIQSLLKRYQ PHRVPSTCCA PVKTKPLSML YVDNGRVLLD HHKDMIVEEC GC, corresponding to amino acids 275-347 of Human Nodal
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特記事項
This product was changed from ascites to tissue culture supernatant on 28th May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
5C3 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab55676の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (3) |
Use at an assay dependent concentration. Predicted molecular weight: 40 kDa.
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IHC-P | (1) |
Use at an assay dependent concentration.
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ICC/IF | (1) |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
特記事項 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 40 kDa. |
IHC-P
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Essential for mesoderm formation and axial patterning during embryonic development. -
関連疾患
Defects in NODAL are the cause of visceral heterotaxy autosomal type 5 (HTX5) [MIM:270100]. A form of visceral heterotaxy, a complex disorder due to disruption of the normal left-right asymmetry of the thoracoabdominal organs. It results in an abnormal arrangement of visceral organs, and a wide variety of congenital defects. Clinical features of visceral heterotaxy autosomal type 5 include situs inversus viscerum or situs ambiguus, congenital heart defect, transposition of the great vessels ventricular septal defect, atrial septal defect, truncuscommunis, and dextrocardia. -
配列類似性
Belongs to the TGF-beta family. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 4838 Human
- Entrez Gene: 18119 Mouse
- Omim: 601265 Human
- SwissProt: Q96S42 Human
- SwissProt: P43021 Mouse
- Unigene: 370414 Human
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別名
- HTX5 antibody
- MGC138230 antibody
- Nodal antibody
see all
画像
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Nodal antibody (ab55676) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human endometrium cancer.
This image was generated using the ascites version of the product.
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Nodal antibody (ab55676) at 1ug/lane + HeLa cell lysate at 25ug/lane.
This image was generated using the ascites version of the product.
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ICC/IF image of ab55676 stained mouse embryonic stem cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55676, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
This image was generated using the ascites version of the product.
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Overlay histogram showing HEK293 cells stained with ab55676 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55676, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HEK293 cells fixed with 100% methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
This image was generated using the ascites version of the product.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (21)
ab55676 は 21 報の論文で使用されています。
- Hong X et al. Biological effects of NODAL on endometrial cancer cells and its underlying mechanisms. Exp Ther Med 21:402 (2021). PubMed: 33717261
- Cave DD et al. Nodal-induced L1CAM/CXCR4 subpopulation sustains tumor growth and metastasis in colorectal cancer derived organoids. Theranostics 11:5686-5699 (2021). PubMed: 33897875
- Harpelunde Poulsen K et al. Influence of Nodal signalling on pluripotency factor expression, tumour cell proliferation and cisplatin-sensitivity in testicular germ cell tumours. BMC Cancer 20:349 (2020). PubMed: 32326899
- Ognibene M & Pezzolo A Roniciclib down-regulates stemness and inhibits cell growth by inducing nucleolar stress in neuroblastoma. Sci Rep 10:12902 (2020). PubMed: 32737364
- Gambichler T et al. Expression of Lefty predicts Merkel cell carcinoma-specific death. J Eur Acad Dermatol Venereol 34:2016-2020 (2020). PubMed: 32022949