Anti-Nocturnin 抗体 (ab22850)

製品の概要

  • 製品名Anti-Nocturnin antibody
  • 製品の詳細
    Rabbit polyclonal to Nocturnin
  • 特異性Although there is a high homology with mouse, this antibody also has the potential to cross react with another mouse protein of similar molecular weight therefore this antibody is not suitable for use in mouse.
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rat, Cow
  • 免疫原

    A region within a synthetic peptide: RLYSALAKTL NSSAASQHPE YLVSPDPEHL EPIDPKELLE ECRAVLHTRP, corresponding to N terminal amino acids 73-122 of Human Nocturnin

  • ポジティブ・コントロール
    • Human lung tissue (IHC-P), Daudi cell lysate (western blot).

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Protein A purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab22850 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 40 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 関連性CCRN4L is highly similar to Nocturnin, a gene identified as a circadian clock regulated gene in Xenopus laevis. This protein and Nocturnin protein share similarity with the C terminal domain of a yeast transcription factor, carbon catabolite repression 4 (CCR4). The mRNA abundance of a similar gene in mouse has been shown to exhibit circadian rhythmicity, which suggests a role for this protein in clock function or as a circadian clock effector.
  • 参照データベース
  • 別名
    • CCR4 carbon catabolite repression 4 like (S. cerevisiae) antibody
    • CCR4 protein homolog antibody
    • CCRN4L antibody
    • NOC antibody

Anti-Nocturnin antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung tissue labelling Nocturnin with ab22850 at 4.0-8.0µg/ml. Positive staining seen in the bronchiole epithelium. Magnification: 400X.
  • Anti-Nocturnin antibody (ab22850) at 4 µg/ml + Daudi cell lysate

    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
    Gel concentration: 12%
  • Paraffin-embedded Human lung tissue. Cells with positive label: epithelial cells of bronchiole (indicated by arrows). Antibody concentration: 4-8µg/ml. Magnification: 400x.

Anti-Nocturnin antibody (ab22850) 使用論文

ab22850 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Mouse Cell lysate - whole cell (3T3 cells (fibroblasts))
Gel Running Conditions Reduced Denaturing (10%)
Loading amount 20 µg
Specification 3T3 cells (fibroblasts)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Abcam user community

Verified customer

投稿 Jun 10 2015

Thank you for getting back to me with further information. I have read your previous questionnaire and the further details that you have provided me with. I find your enquiry most interesting. As I can see you are ONLY detecting the correctly sized...

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Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. I have read your technical questionnaire and I have a few comments. You mention that this antibody has been detecting bands other than ove...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"