The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Nociceptin: Ligand of the opioid receptor-like receptor OPRL1. It may act as a transmitter in the brain by modulating nociceptive and locomotor behavior. May be involved in neuronal differentiation and development. Nocistatin: Blocks nociceptin action in pain transmission by inhibiting nociceptin-induced hyperalgesia and allodynia. Orphanin FQ2: Has potent analgesic activity.
Predominantly expressed in the brain and spinal cord. Also expressed and secreted by peripheral blood neutrophils following degranulation.
Belongs to the opioid neuropeptide precursor family.
Specific enzymatic cleavages at paired basic residues probably yield other active peptides besides nociceptin. The N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing.
Confocal images showing Nociceptin (OFQ) (red), GnRH (green) and their colocalization (yellow) in the sheep preoptic area and hypothalamus.
A-C: Low power view of the preoptic area at the level of the organum vasculosum of the lamina terminalis (OVLT) showing OFQ and GnRH cells, and their colocalization (e.g., white arrows). Bar = 100µm. D-F: High power images of a double-labeled OFQ/GnRH cell; note that the immunofluorescence for OFQ has a punctateappearance in the cytoplasm as opposed to the more diffuse appearance of the GnRH signal. Bar 26 = 20µm. G-I: Single-labeled OFQ cells (e.g., red arrow) in the arcuate nucleus. Note that many GnRH fibers coursing through this region are also OFQ-positive (e.g., white arrows). 3v = third ventricle. Bar = 100µm.