Anti-Nociceptin 抗体 (ab100880)
Key features and details
- Rabbit polyclonal to Nociceptin
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Nociceptin antibody
Nociceptin 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Nociceptin -
由来種
Rabbit -
アプリケーション
適用あり: IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Rabbit, Horse, Dog, Pig -
免疫原
Synthetic peptide:
FGGFTGARKSARKLANQ
, corresponding to internal sequence amino acids 130-146 of Human Nociceptin -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.02% Sodium azide
Constituent: Whole serum -
Concentration information loading...
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精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab100880の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P |
1/2000.
|
特記事項 |
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IHC-P
1/2000. |
ターゲット情報
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機能
Nociceptin: Ligand of the opioid receptor-like receptor OPRL1. It may act as a transmitter in the brain by modulating nociceptive and locomotor behavior. May be involved in neuronal differentiation and development.
Nocistatin: Blocks nociceptin action in pain transmission by inhibiting nociceptin-induced hyperalgesia and allodynia.
Orphanin FQ2: Has potent analgesic activity. -
組織特異性
Predominantly expressed in the brain and spinal cord. Also expressed and secreted by peripheral blood neutrophils following degranulation. -
配列類似性
Belongs to the opioid neuropeptide precursor family. -
翻訳後修飾
Specific enzymatic cleavages at paired basic residues probably yield other active peptides besides nociceptin.
The N-terminal domain contains 6 conserved cysteines thought to be involved in disulfide bonding and/or processing. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 5368 Human
- Omim: 601459 Human
- SwissProt: Q13519 Human
- SwissProt: P55791 Pig
- Unigene: 88218 Human
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別名
- N/OFQ antibody
- N23K antibody
- N23K/N27K antibody
see all
画像
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Dual labeling of Nociceptin (green) and enkephalin (red). 7 μm paraffin sections of human brain tissue were cut and floated onto positively charged slides. After being dewaxed and microwaved in 10mM citric acid (pH 6.0) for 20 minutes at 600W, sections were incubated with the rabbit polyclonal antisera overnight at 4°C, primary antibody was detected using biotinylated goat anti-rabbit IgG followed by incubation with peroxidase-conjugated avidin.
Binding of the primary antibody was visualized using 3-amino-9-ethylcarbazole (AEC) in acetate buffer. Afterwards, sections were rinsed, counterstained with Mayer`s hematoxylin and mounted in Vectamount mounting medium.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (0)
ab100880 は論文での使用が確認できていません。