製品の概要

  • 製品名Anti-nNOS (neuronal) antibody
    nNOS (neuronal) 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to nNOS (neuronal)
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Rat, Human
    交差が予測される動物種: Mouse, Rabbit, Lizard
  • 免疫原

    Synthetic peptide:

    IAFIEESKKDTDEVFSS

    , corresponding to C terminal amino acids 1418-1434 of Human nNOS (neuronal)

  • ポジティブ・コントロール
    • Rat brain tissue. Rat medulla oblongata tissue. whole cell lysate from MCF7, SMMC, SW620 and COLO 320 cells.
  • 特記事項The immunogen sequence is identical to the related rat sequence

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab95436 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 161 kDa.
IHC-P Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 機能Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.
  • 組織特異性Isoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected in testis, skeletal muscle, lung, and kidney, at low levels in the brain, but not in the heart and adrenal gland.
  • 配列類似性Belongs to the NOS family.
    Contains 1 FAD-binding FR-type domain.
    Contains 1 flavodoxin-like domain.
    Contains 1 PDZ (DHR) domain.
  • ドメインThe PDZ domain in the N-terminal part of the neuronal isoform participates in protein-protein interaction, and is responsible for targeting nNos to synaptic membranes in muscles. Mediates interaction with VAC14.
  • 翻訳後修飾Ubiquitinated; mediated by STUB1/CHIP in the presence of Hsp70 and Hsp40 (in vitro).
  • 細胞内局在Cell membrane > sarcolemma. Cell projection > dendritic spine. In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 2310005C01Rik antibody
    • BNOS antibody
    • Constitutive NOS antibody
    • EC 1.14.13.39 antibody
    • IHPS 1 antibody
    • IHPS1 antibody
    • N-NOS antibody
    • NC-NOS antibody
    • neuronal Nitric Oxide Synthase antibody
    • Neuronal NOS antibody
    • Nitric oxide synthase , neuronal, included antibody
    • Nitric oxide synthase 1 (neuronal) antibody
    • Nitric oxide synthase 1 antibody
    • Nitric oxide synthase, brain antibody
    • Nitric oxide synthase, penile neuronal, included antibody
    • NNOS antibody
    • NO antibody
    • NOS 1 antibody
    • NOS antibody
    • NOS type I antibody
    • NOS-I antibody
    • NOS1 antibody
    • NOS1_HUMAN antibody
    • Peptidyl-cysteine S-nitrosylase NOS1 antibody
    see all

Anti-nNOS (neuronal) antibody 画像

  • ab95436 at 2µg/ml staining NOS in rat brain tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). A heat mediated antigen retrieval step was performed.
  • All lanes : Anti-nNOS (neuronal) antibody (ab95436) at 1 µg/ml

    Lane 1 : Lysate prepared from rat brain tissue.
    Lane 2 : Lysate prepared from rat brain tissue.
    Lane 3 : Lysate prepared from rat medulla oblongata tissue.
    Lane 4 : Whole cell lysate prepared from MCF7 cells.
    Lane 5 : Whole cell lysate prepared from SMMC cells.
    Lane 6 : Whole cell lysate prepared from SW620 cells.
    Lane 7 : Whole cell lysate prepared from Colo320 cells.


    Predicted band size : 161 kDa

Anti-nNOS (neuronal) antibody (ab95436) 使用論文

ab95436 has not yet been referenced specifically in any publications.

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