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Our Abpromise guarantee covers the use of ab3342 in the following tested applications.
|ICC||Use at an assay dependent concentration.|
By Western blot, this antibody detects: An ~135 kDa protein representing Mouse macrophage iNOS. An ~155 kDa protein representing rat, bovine and porcine bNOS. An ~140 kDa protein representing porcine and bovine eNOS.
|IHC-P||Use a concentration of 5 µg/ml.|
ab3342 staining nNOS (neuronal) in Drosophila optic nerve by Immunohistochemistry (Frozen sections)
IHC image of ab3342 staining nNOS (neuronal) in Human Testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab3342, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.