製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to NNMT
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide: MESGFTSKDT YLSHFNPRDY LEKYYKFGSR HSAESQILKH LLKNLFKIFC, corresponding to amino acids 1-50 of Human NNMT

  • ポジティブ・コントロール
    • HepG2 cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab58743 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 29 kDa (predicted molecular weight: 30 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/62500.

ターゲット情報

Anti-NNMT antibody 画像

  • Anti-NNMT antibody (ab58743) at 1/2.5 dilution + HepG2 cell lysate at 10 µg with skim milk/PBS buffer at 5 %

    Secondary
    HRP conjugated anti-Rabbit IgG at 1: 50,000 - 100,000

    Predicted band size : 30 kDa
    Observed band size : 29 kDa (why is the actual band size different from the predicted?)

プロトコール

Anti-NNMT antibody (ab58743) 使用論文

This product has been referenced in:
  • Jung J  et al. Nicotinamide metabolism regulates glioblastoma stem cell maintenance. JCI Insight 2:N/A (2017). Read more (PubMed: 28515364) »
  • Fedorowicz A  et al. Activation of the nicotinamide N-methyltransferase (NNMT)-1-methylnicotinamide (MNA) pathway in pulmonary hypertension. Respir Res 17:108 (2016). IHC-Fr ; Rat . Read more (PubMed: 27581040) »

See all 3 Publications for this product

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