Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (hEK293)
Total protein in input
300 µg
Immuno-precipitation step
Other - protein G dynabeads
Specification
hEK293
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: AFC high salt
Western Blot antibody
Western Blot antibody
None used
Additional data
Additional Notes
This immunoprecipitation was followed by mass spectrometry identification of the immunoprecipitated peptides. The same immunoprecipitation protocol was carried out with an unrelated antibody control. After removal of background contaminants the identified peptides were sorted according to the highest number of spectral counts identified per protein. If the intended antigen is at the top of the list, the antibody is judged to be very good. If the intended antigen is lower on the list but tho proteins above are known components of the antigen's complex, the antibody is also judged to be very good. The table shows the proteins with the highest number of spectral counts identified (after removal of background contaminants).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Edyta Marcon
Verified customer
投稿 Jun 26 2015