製品の概要

  • 製品名Anti-nmt55 / p54nrb antibody
    nmt55 / p54nrb 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to nmt55 / p54nrb
  • アプリケーション適用あり: ICC/IF, IHC-P, WB, IPmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • 免疫原

    Synthetic peptide corresponding to a region between residues 350 and 400 of human nmt55/ p54nrb (NP_031389.3).

  • ポジティブ・コントロール
    • Whole cell lysates from HeLa and 293T cells.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab70335 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 54 kDa).
IP Use at 1-4 µg/mg of lysate.

ターゲット情報

  • 機能DNA- and RNA binding protein, involved in several nuclear processes. Binds the conventional octamer sequence in double stranded DNA. Also binds single-stranded DNA and RNA at a site independent of the duplex site (By similarity). Involved in pre-mRNA splicing, probably as an heterodimer with SFPQ. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. The SFPQ-NONO heteromer may be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1. The SFPQ-NONO heteromer may be involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends. In vitro, the complex strongly stimulates DNA end joining, binds directly to the DNA substrates and cooperates with the Ku70/G22P1-Ku80/XRCC5 (Ku) dimer to establish a functional preligation complex. Nono is involved in transcriptional regulation. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity. NONO binds to an enhancer element in long terminal repeats of endogenous intracisternal A particles (IAPs) and activates transcription.
  • 組織特異性Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Also found in a number of breast tumor cell lines.
  • 関連疾患Note=A chromosomal aberration involving NONO may be a cause of papillary renal cell carcinoma (PRCC). Translocation t(X;X)(p11.2;q13.1) with TFE3.
  • 配列類似性Contains 2 RRM (RNA recognition motif) domains.
  • 翻訳後修飾The N-terminus is blocked.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 52 kDa subunit antibody
    • 54 kDa nuclear RNA and DNA binding protein antibody
    • 54 kDa nuclear RNA- and DNA-binding protein antibody
    • 55 kDa nuclear protein antibody
    • DNA binding p52/p100 complex 52 kDa subunit antibody
    • DNA-binding p52/p100 complex antibody
    • NMT 55 antibody
    • NMT55 antibody
    • Non Pou domain containing octamer (ATGCAAAT) binding protein antibody
    • Non POU domain containing octamer binding antibody
    • Non POU domain containing octamer binding protein antibody
    • Non-POU domain-containing octamer-binding protein antibody
    • Nono antibody
    • NonO protein antibody
    • NONO_HUMAN antibody
    • NRB 54 antibody
    • NRB antibody
    • NRB54 antibody
    • Nuclear RNA binding protein 54kD antibody
    • P54 antibody
    • p54(nrb) antibody
    • p54nrb antibody
    • PPP1R114 antibody
    • Protein phosphatase 1 regulatory subunit 114 antibody
    see all

Anti-nmt55 / p54nrb antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma (left) and mouse squamous cell carcinoma (right) tissues labelling nmt55 / p54nrb with ab70335 at 1/1000 (0.2µg/ml) and 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-nmt55 / p54nrb antibody (ab70335) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg


    Predicted band size : 54 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
  • Detection of Human nmt55 / p54nrb by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 1/4 of IP loaded) using ab70335 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent WB detection. Lane 2 represents control IgG IP.
  • ICC/IF image of ab70335 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab42905, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab70335 staining in human normal kidney formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70335, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-nmt55 / p54nrb antibody (ab70335) at 0.1 µg/ml

    Lane 1 : NIH3T3 whole cell lysate
    Lane 2 : TCMK-1 whole cell lysate
    Lane 3 : 4T1 whole cell lysate
    Lane 4 : CT26.WT whole cell lysate
    Lane 5 : C6 whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique

    Predicted band size : 54 kDa


    Exposure time : 30 seconds

Anti-nmt55 / p54nrb antibody (ab70335) 使用論文

This product has been referenced in:
  • Innes BT  et al. Non-catalytic participation of the Pin1 peptidyl-prolyl isomerase domain in target binding. Front Physiol 4:18 (2013). WB ; Human . Read more (PubMed: 23407864) »
  • Ong SA  et al. Rasd1 Modulates the Coactivator Function of NonO in the Cyclic AMP Pathway. PLoS One 6:e24401 (2011). IP ; Human . Read more (PubMed: 21915321) »

See all 4 Publications for this product

Product Wall

Application Immunoprecipitation
Sample Human Cell lysate - whole cell (hEK293)
Total protein in input 300 µg
Immuno-precipitation step Other - protein G dynabeads
Specification hEK293
Username

Dr. Edyta Marcon

Verified customer

投稿 Jun 26 2015

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (10 % GEL, SDS)
Sample Human Cell lysate - whole cell (HEK293)
Specification HEK293
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

投稿 Apr 29 2014

Application Western blot
Sample Mouse Tissue lysate - whole (mouse testis)
Loading amount 50 µg
Specification mouse testis
Treatment Irradiation - 20 gy
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Dec 07 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - nuclear (HEK293)
Loading amount 100 µg
Specification HEK293
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Dec 06 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"