製品の概要

  • 製品名Anti-nmt55 / p54nrb antibody
    nmt55 / p54nrb 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to nmt55 / p54nrb
  • アプリケーション適用あり: WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Cow, Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human nmt55/ p54nrb.

    (Peptide available as ab45358.)

  • ポジティブ・コントロール
    • ab45359 gave a positive result in the following whole cell lysates: Hela, A431, HEK293, HepG2, MCF7, U20S. ab45359 also gave a positive result in Hela Nuclear lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab45359 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 54 kDa).
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能DNA- and RNA binding protein, involved in several nuclear processes. Binds the conventional octamer sequence in double stranded DNA. Also binds single-stranded DNA and RNA at a site independent of the duplex site (By similarity). Involved in pre-mRNA splicing, probably as an heterodimer with SFPQ. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. The SFPQ-NONO heteromer may be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1. The SFPQ-NONO heteromer may be involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends. In vitro, the complex strongly stimulates DNA end joining, binds directly to the DNA substrates and cooperates with the Ku70/G22P1-Ku80/XRCC5 (Ku) dimer to establish a functional preligation complex. Nono is involved in transcriptional regulation. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity. NONO binds to an enhancer element in long terminal repeats of endogenous intracisternal A particles (IAPs) and activates transcription.
  • 組織特異性Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Also found in a number of breast tumor cell lines.
  • 関連疾患Note=A chromosomal aberration involving NONO may be a cause of papillary renal cell carcinoma (PRCC). Translocation t(X;X)(p11.2;q13.1) with TFE3.
  • 配列類似性Contains 2 RRM (RNA recognition motif) domains.
  • 翻訳後修飾The N-terminus is blocked.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 52 kDa subunit antibody
    • 54 kDa nuclear RNA and DNA binding protein antibody
    • 54 kDa nuclear RNA- and DNA-binding protein antibody
    • 55 kDa nuclear protein antibody
    • DNA binding p52/p100 complex 52 kDa subunit antibody
    • DNA-binding p52/p100 complex antibody
    • NMT 55 antibody
    • NMT55 antibody
    • Non Pou domain containing octamer (ATGCAAAT) binding protein antibody
    • Non POU domain containing octamer binding antibody
    • Non POU domain containing octamer binding protein antibody
    • Non-POU domain-containing octamer-binding protein antibody
    • Nono antibody
    • NonO protein antibody
    • NONO_HUMAN antibody
    • NRB 54 antibody
    • NRB antibody
    • NRB54 antibody
    • Nuclear RNA binding protein 54kD antibody
    • P54 antibody
    • p54(nrb) antibody
    • p54nrb antibody
    • PPP1R114 antibody
    • Protein phosphatase 1 regulatory subunit 114 antibody
    see all

Anti-nmt55 / p54nrb antibody 画像

  • All lanes : Anti-nmt55 / p54nrb antibody (ab45359) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
    Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : HEK293 Human embryonic kidney cell line Whole Cell Lysate
    Lane 5 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 7 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 54 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab45359 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab45359, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

  • IHC image of nmt55/p54nrb staining in human breast carcinoma FFPE section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab45359, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-nmt55 / p54nrb antibody (ab45359) 使用論文

This product has been referenced in:
  • Li Z  et al. The long noncoding RNA THRIL regulates TNFa expression through its interaction with hnRNPL. Proc Natl Acad Sci U S A 111:1002-7 (2014). WB ; Human . Read more (PubMed: 24371310) »

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 15 µg
Gel Running Conditions Reduced Denaturing (12.5%)
Sample Mouse Cell lysate - whole cell (Murine microglial cells)
Specification Murine microglial cells
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Lucy

Verified customer

投稿 Apr 09 2014

DISCOUNT CODE: ********* Expiration date: ********* I am very pleased to hear you would like to accept our offer and test ab79340 in bovine tissue. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Cow Cell lysate - whole cell (chondrocytes)
Loading amount 20 µg
Specification chondrocytes
Gel Running Conditions Reduced Denaturing (12)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Dr. Nazish Ahmed

Verified customer

投稿 Aug 04 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"