Rabbit polyclonal to NME4 - C-terminal
Synthetic peptide from the C terminal region of human NME4 conjugated to KLH
Jurkat cell lysate and human breast carcinoma tissue.
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Concentration information loading...
Protein G purified
ab71623 is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
1/50 - 1/100.
Major role in the synthesis of nucleoside triphosphates other than ATP.
Widely distributed. Found at very high levels in prostate, heart, liver, small intestine, and skeletal muscle tissues, and in low amounts in the brain and in blood leukocytes.
Belongs to the NDK family.
Mitochondrion intermembrane space.
Information by UniProt
metastatic inhibition factor NM23H4 antibody
Western blot - NME4 antibody - C-terminal (ab71623)
Anti-NME4 antibody - C-terminal (ab71623) at 1/100 dilution + Jurkat cell lysate at 12.5 µg
Predicted band size: 21 kDa Observed band size: 21 kDa Additional bands at: 40 kDa, 60 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - NME4 antibody - C-terminal (ab71623)
ab71623 at 1/50 dilution, staining NME4 in human breast carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and AEC staining.
has not yet been referenced specifically in any publications.
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