N methyl D asparate receptor channel subunit epsilon 2 antibody
N methyl D aspartate receptor subtype 2B antibody
N methyl D aspartate receptor subunit 2B antibody
N methyl D aspartate receptor subunit 3 antibody
N-methyl D-aspartate receptor subtype 2B antibody
N-methyl-D-aspartate receptor subunit 3 antibody
NMDA NR2B antibody
NMDA R2B antibody
Western blot - Anti-NMDAR2B (phospho Y1252) antibody (ab18532)This image is courtesy of Chris Anderson, Wellcome Trust Sanger Institute, United Kingdom
Lanes 1 - 3 : Anti-NMDAR2B (phospho Y1252) antibody (ab18532) at 3 µg/ml Lane 4 : NR2B antibody (positive control)
Lane 1 : mouse cortical extract with Mouse NMDAR2B (unmodified ) peptide (ab19637) at 2 µg/ml Lane 2 : mouse cortical extract with Mouse NMDAR2B (phospho Y1252) peptide (ab19636) at 2 µg/ml Lane 3 : mouse cortical extract Lane 4 : mouse cortical extract
Lysates/proteins at 20 µg per lane.
Predicted band size : 166 kDa Observed band size : 166 kDa Additional bands at : 115 kDa. We are unsure as to the identity of these extra bands.
This image is courtesy of Chris Anderson, Wellcome Trust Sanger Institute, United Kingdom
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-NMDAR2B (phospho Y1252) antibody (ab18532)This image is courtesy of an abreview submitted by Sophie Pezet, Laboratoire de Neurobiologie, France
IHC-FoFr image of NMDAR2B(pY1252) staining on mouse brain sections (A) Hypothalamic paraventricular nucleus and (B) Cerebral cortex using ab18532(1:100). The sections used came from animals perfused fixed with Paraformaldehyde 4% with 15% of a solution of saturated picric acid, in phosphate buffer 0.1M. Following postfixation in the same fixative overnight, the brains were cryoprotected in sucrose 30% overnight. Brains were then cut using a cryostat and the immunostainings were performed using the ‘free floating’ technique.
Delint-Ramirez I et al. In vivo composition of NMDA receptor signaling complexes differs between membrane subdomains and is modulated by PSD-95 and PSD-93. J Neurosci30:8162-70 (2010).
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