Anti-NMDAR2B (phospho S1303) 抗体 [EP1858Y] (ab81271)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1858Y] to NMDAR2B (phospho S1303)
- Suitable for: Dot blot, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-NMDAR2B (phospho S1303) antibody [EP1858Y]
NMDAR2B 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP1858Y] to NMDAR2B (phospho S1303) -
由来種
Rabbit -
アプリケーション
適用あり: Dot blot, WBmore details
適用なし: IHC-P or IP -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Human, mouse and rat brain tissue lysates.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP1858Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab81271の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Dot blot |
1/1000.
|
|
WB | (1) |
1/1000 - 1/5000. Predicted molecular weight: 166 kDa.
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特記事項 |
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Dot blot
1/1000. |
WB
1/1000 - 1/5000. Predicted molecular weight: 166 kDa. |
ターゲット情報
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機能
NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. -
組織特異性
Primarily found in the fronto-parieto-temporal cortex and hippocampus pyramidal cells, lower expression in the basal ganglia. -
配列類似性
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2B/GRIN2B subfamily. -
細胞内局在
Cell membrane. Cell junction > synapse > postsynaptic cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 2904 Human
- Entrez Gene: 14812 Mouse
- Entrez Gene: 24410 Rat
- Omim: 138252 Human
- SwissProt: Q13224 Human
- SwissProt: Q01097 Mouse
- SwissProt: Q00960 Rat
- Unigene: 654430 Human
see all -
別名
- AW490526 antibody
- EIEE27 antibody
- Glutamate [NMDA] receptor subunit epsilon 2 antibody
see all
画像
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All lanes : Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271) at 1/5000 dilution
Lane 1 : Rat brain tissue lysate
Lane 2 : Rat brain tissue lysate. The membrane was incubated with alkaline phosphatase.
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 166 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271) at 1/5000 dilution
Lane 1 : Human brain tissue lysate
Lane 2 : Human brain tissue lysate. The membrane was incubated with alkaline phosphatase.
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 166 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
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Dot blot analysis of NMDAR2B (phospho S1303) phospho peptide (Lane 1) and NMDAR2B non-phospho peptide (Lane 2) labelling NMDAR2B (phospho S1303) with ab81271 at a dilution of 1/1000. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/20,000 dilution. Blocking buffer: 5% NFDM/TBST. Dilution buffer: 5% NFDM /TBST.
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All lanes : Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271) at 1/5000 dilution
Lane 1 : Mouse brain lysates (untreated)
Lane 2 : Mouse brain lysates (treated with AP)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP labelled IgG at 1/2000 dilution
Predicted band size: 166 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (27)
ab81271 は 27 報の論文で使用されています。
- Wang XS et al. Activation of GIPR Exerts Analgesic and Anxiolytic-Like Effects in the Anterior Cingulate Cortex of Mice. Front Endocrinol (Lausanne) 13:887238 (2022). PubMed: 35712239
- Sun Z et al. Casein kinase 2 attenuates brain injury induced by intracerebral hemorrhage via regulation of NR2B phosphorylation. Front Cell Neurosci 16:911973 (2022). PubMed: 35928572
- Liu B et al. BHF177 Suppresses Diabetic Neuropathic Pain by Blocking PKC/CaMKII/ERK1/2/CREB Signaling Pathway through Activating GABAB Receptor. Oxid Med Cell Longev 2022:4661519 (2022). PubMed: 36439691
- Liu S et al. Icaritin Alleviates Glutamate-Induced Neuronal Damage by Inactivating GluN2B-Containing NMDARs Through the ERK/DAPK1 Pathway. Front Neurosci 15:525615 (2021). PubMed: 33692666
- Gwon DH et al. NFAT5 Deficiency Alleviates Formalin-Induced Inflammatory Pain Through mTOR. Int J Mol Sci 22:N/A (2021). PubMed: 33806698