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RabMAb

Anti-NMDAR2B (phospho S1303) 抗体 [EP1858Y] (ab81271)

製品の概要

  • 製品名
    Anti-NMDAR2B (phospho S1303) antibody [EP1858Y]
    NMDAR2B 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1858Y] to NMDAR2B (phospho S1303)
  • 特異性
    ab81271 detects NMDAR2B when phosphorylated at serine 1303
  • アプリケーション
    適用あり: WB, ICCmore details
    適用なし: IHC-P or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A phospho specific peptide corresponding to residues surrounding serine 1303 of human NMDAR2B.

  • ポジティブ・コントロール
    • Mouse brain lysates
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

     

    Alternative versions available:

    Anti-NMDAR2B (phospho S1303) antibody (HRP) [EP1858Y] (ab196974)

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab81271 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/5000. Predicted molecular weight: 166 kDa.
ICC 1/100.
  • 追加情報
    Is unsuitable for IHC-P or IP.
  • ターゲット情報

    • 機能
      NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine.
    • 組織特異性
      Primarily found in the fronto-parieto-temporal cortex and hippocampus pyramidal cells, lower expression in the basal ganglia.
    • 配列類似性
      Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2B/GRIN2B subfamily.
    • 細胞内局在
      Cell membrane. Cell junction > synapse > postsynaptic cell membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • AW490526 antibody
      • EIEE27 antibody
      • Glutamate [NMDA] receptor subunit epsilon 2 antibody
      • Glutamate [NMDA] receptor subunit epsilon-2 antibody
      • Glutamate Receptor Ionotropic N Methyl D Aspartate 2B antibody
      • Glutamate Receptor Ionotropic N Methyl D Aspartate subunit 2B antibody
      • Glutamate receptor ionotropic NMDA2B antibody
      • Glutamate receptor subunit epsilon 2 antibody
      • Glutamate receptor, ionotropic, NMDA2B (epsilon 2) antibody
      • GRIN 2B antibody
      • GRIN2B antibody
      • hNR 3 antibody
      • hNR3 antibody
      • MGC142178 antibody
      • MGC142180 antibody
      • MRD6 antibody
      • N methyl D asparate receptor channel subunit epsilon 2 antibody
      • N methyl D aspartate receptor subtype 2B antibody
      • N methyl D aspartate receptor subunit 2B antibody
      • N methyl D aspartate receptor subunit 3 antibody
      • N-methyl D-aspartate receptor subtype 2B antibody
      • N-methyl-D-aspartate receptor subunit 3 antibody
      • NMDA NR2B antibody
      • NMDA R2B antibody
      • NMDAR2B antibody
      • NMDE2 antibody
      • NMDE2_HUMAN antibody
      • NME2 antibody
      • NR2B antibody
      • NR3 antibody
      see all

    Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] 画像

    • All lanes : Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271) at 1/5000 dilution

      Lane 1 : mouse brain lysates (untreated)
      Lane 2 : mouse brain lysates (treated with AP)

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit HRP labelled IgG at 1/2000 dilution

      Predicted band size : 166 kDa
      Observed band size : 180 kDa (why is the actual band size different from the predicted?)

    Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271) 使用論文

    This product has been referenced in:
    • Wang Y  et al. Propofol-induced downregulation of NR2B membrane translocation in hippocampus and spatial memory deficits of neonatal mice. Brain Behav 7:e00734 (2017). Read more (PubMed: 28729940) »
    • Wang XQ  et al. Differential roles of hippocampal glutamatergic receptors in neuropathic anxiety-like behavior after partial sciatic nerve ligation in rats. BMC Neurosci 16:14 (2015). WB, IHC . Read more (PubMed: 25884414) »

    See all 5 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (brain)
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native)
    Loading amount
    15 µg
    Specification
    brain
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    投稿 Jun 20 2017

    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (brain)
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native) (gel 8%)
    Loading amount
    15 µg
    Treatment
    brain
    Specification
    brain
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    投稿 Apr 21 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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