Rabbit polyclonal to NMDAR2B
Mouse, Chicken, Cow, Dog, Human, Non human primates
Synthetic peptide from the N-terminus of rat NMDAR2B.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Constituents: 50% Glycerol, 100µg/ml BSA, 150mM Sodium chloride, 10mM HEPES, pH 7.5
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Detects a band of approximately 180 kDa (predicted molecular weight: 166 kDa).
NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine.
Primarily found in the fronto-parieto-temporal cortex and hippocampus pyramidal cells, lower expression in the basal ganglia.
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2B/GRIN2B subfamily.
Cell membrane. Cell junction > synapse > postsynaptic cell membrane.
Information by UniProt
Glutamate [NMDA] receptor subunit epsilon 2 antibody
Western blot - NMDAR2B antibody (ab73001)
Anti-NMDAR2B antibody (ab73001) at 1/1000 dilution + rat hippocampal lysate at 10 µg
Predicted band size:
Observed band size:
180 kDa (
why is the actual band size different from the predicted?
Additional bands at:
<68 kDa, 120 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - Anti-NMDAR2B antibody (ab73001)
ab73001 diluted 1/500 staining NMDAR2B on 14DIV cultured rat cortical neurons fixed in methanol at - 20
oC for 10 minutes. Sections were also labelled with an anti-PSD95 antibody in red.
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