保存方法 Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
バッファー Preservative: None
Constituents: 50% Glycerol, 100µg/ml BSA, 150mM Sodium chloride, 10mM HEPES, pH 7.5
Concentration information loading...
精製度 Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Detects a band of approximately 180 kDa (predicted molecular weight: 166 kDa).
機能 NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine.
組織特異性 Primarily found in the fronto-parieto-temporal cortex and hippocampus pyramidal cells, lower expression in the basal ganglia.
配列類似性 Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2B/GRIN2B subfamily.
細胞内局在 Cell membrane. Cell junction > synapse > postsynaptic cell membrane.
Information by UniProt
Glutamate [NMDA] receptor subunit epsilon 2 antibody
Anti-NMDAR2B antibody 画像
Western blot - NMDAR2B antibody (ab73001)
Anti-NMDAR2B antibody (ab73001) at 1/1000 dilution + rat hippocampal lysate at 10 µg Predicted band size : 166 kDa Observed band size : 180 kDa ( why is the actual band size different from the predicted?) Additional bands at : 120 kDa,<68 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - Anti-NMDAR2B antibody (ab73001)
ab73001 diluted 1/500 staining NMDAR2B on 14DIV cultured rat cortical neurons fixed in methanol at - 20
oC for 10 minutes. Sections were also labelled with an anti-PSD95 antibody in red.
Anti-NMDAR2B antibody (ab73001) 使用論文
This product has been referenced in:
et al. Synaptic and cognitive improvements by inhibition of 2-AG metabolism are through upregulation of microRNA-188-3p in a mouse model of Alzheimer's disease. J Neurosci 34:14919-33 (2014).
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