製品の概要

  • 製品名Anti-NMDAR2B antibody
    NMDAR2B 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to NMDAR2B
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Dog, Xenopus laevis
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1350 - 1450 of Rat NMDAR2B.

    (Peptide available as ab71177.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following tissue lysates: Brain (Human), Brain (Mouse), Brain (Rat), Hippocampus (Mouse), Rat Hippocampus

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab71178 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 180 kDa (predicted molecular weight: 166 kDa).Can be blocked with Human NMDAR2B peptide (ab71177).
IP Use at an assay dependent concentration.

ターゲット情報

  • 機能NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine.
  • 組織特異性Primarily found in the fronto-parieto-temporal cortex and hippocampus pyramidal cells, lower expression in the basal ganglia.
  • 配列類似性Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2B/GRIN2B subfamily.
  • 細胞内局在Cell membrane. Cell junction > synapse > postsynaptic cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AW490526 antibody
    • EIEE27 antibody
    • Glutamate [NMDA] receptor subunit epsilon 2 antibody
    • Glutamate [NMDA] receptor subunit epsilon-2 antibody
    • Glutamate Receptor Ionotropic N Methyl D Aspartate 2B antibody
    • Glutamate Receptor Ionotropic N Methyl D Aspartate subunit 2B antibody
    • Glutamate receptor ionotropic NMDA2B antibody
    • Glutamate receptor subunit epsilon 2 antibody
    • Glutamate receptor, ionotropic, NMDA2B (epsilon 2) antibody
    • GRIN 2B antibody
    • GRIN2B antibody
    • hNR 3 antibody
    • hNR3 antibody
    • MGC142178 antibody
    • MGC142180 antibody
    • MRD6 antibody
    • N methyl D asparate receptor channel subunit epsilon 2 antibody
    • N methyl D aspartate receptor subtype 2B antibody
    • N methyl D aspartate receptor subunit 2B antibody
    • N methyl D aspartate receptor subunit 3 antibody
    • N-methyl D-aspartate receptor subtype 2B antibody
    • N-methyl-D-aspartate receptor subunit 3 antibody
    • NMDA NR2B antibody
    • NMDA R2B antibody
    • NMDAR2B antibody
    • NMDE2 antibody
    • NMDE2_HUMAN antibody
    • NME2 antibody
    • NR2B antibody
    • NR3 antibody
    see all

Anti-NMDAR2B antibody 画像

  • All lanes : Anti-NMDAR2B antibody (ab71178) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Brain (Mouse) Tissue Lysate
    Lane 3 : Brain (Rat) Tissue Lysate
    Lane 4 : Hippocampus (Mouse) Tissue Lysate
    Lane 5 : Rat Hippocampus Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 166 kDa
    Observed band size : 180 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes
  • NMDAR2B was immunoprecipitated using 0.5mg Mouse Brain whole tissue lysate, 5µg of Rabbit polyclonal to NMDAR2B and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain whole tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab71178.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 180kDa: NMDAR2B.

Anti-NMDAR2B antibody (ab71178) 使用論文

ab71178 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Brain)
Specification Brain
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Username

Dr. Sophie Pezet

Verified customer

投稿 Sep 07 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"