N methyl D asparate receptor channel subunit epsilon 2 antibody
N methyl D aspartate receptor subtype 2B antibody
N methyl D aspartate receptor subunit 2B antibody
N methyl D aspartate receptor subunit 3 antibody
N-methyl D-aspartate receptor subtype 2B antibody
N-methyl-D-aspartate receptor subunit 3 antibody
NMDA NR2B antibody
NMDA R2B antibody
Western blot - NMDAR2B antibody (ab71178)
All lanes : Anti-NMDAR2B antibody (ab71178) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466) Lane 2 : Brain (Mouse) Tissue Lysate Lane 3 : Brain (Rat) Tissue Lysate Lane 4 : Hippocampus (Mouse) Tissue Lysate Lane 5 : Rat Hippocampus Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
NMDAR2B was immunoprecipitated using 0.5mg Mouse Brain whole tissue lysate, 5µg of Rabbit polyclonal to NMDAR2B and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain whole tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation. Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab71178. Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697). Band: 180kDa: NMDAR2B.