製品の概要

  • 製品名Anti-NMDAR2B antibody
    NMDAR2B 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to NMDAR2B
  • アプリケーション適用あり: WB, IHC-FoFr, ICC/IF, IPmore details
  • 種交差性
    交差種: Mouse, Rat, Chicken, Human, Xenopus laevis
    交差が予測される動物種: Dog, Zebrafish
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1450 to the C-terminus of Rat NMDAR2B.

    (Peptide available as ab71176.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following tissue lysates: Brain (Human), Brain (Mouse), Brain (Rat), Hippocampus (Mouse), Rat Hippocampus, Human Hippocampus, Human Amygdala.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab65783 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 180 kDa (predicted molecular weight: 166 kDa).
IHC-FoFr Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 µg/ml.
IP Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine.
  • 組織特異性Primarily found in the fronto-parieto-temporal cortex and hippocampus pyramidal cells, lower expression in the basal ganglia.
  • 配列類似性Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2B/GRIN2B subfamily.
  • 細胞内局在Cell membrane. Cell junction > synapse > postsynaptic cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AW490526 antibody
    • EIEE27 antibody
    • Glutamate [NMDA] receptor subunit epsilon 2 antibody
    • Glutamate [NMDA] receptor subunit epsilon-2 antibody
    • Glutamate Receptor Ionotropic N Methyl D Aspartate 2B antibody
    • Glutamate Receptor Ionotropic N Methyl D Aspartate subunit 2B antibody
    • Glutamate receptor ionotropic NMDA2B antibody
    • Glutamate receptor subunit epsilon 2 antibody
    • Glutamate receptor, ionotropic, NMDA2B (epsilon 2) antibody
    • GRIN 2B antibody
    • GRIN2B antibody
    • hNR 3 antibody
    • hNR3 antibody
    • MGC142178 antibody
    • MGC142180 antibody
    • MRD6 antibody
    • N methyl D asparate receptor channel subunit epsilon 2 antibody
    • N methyl D aspartate receptor subtype 2B antibody
    • N methyl D aspartate receptor subunit 2B antibody
    • N methyl D aspartate receptor subunit 3 antibody
    • N-methyl D-aspartate receptor subtype 2B antibody
    • N-methyl-D-aspartate receptor subunit 3 antibody
    • NMDA NR2B antibody
    • NMDA R2B antibody
    • NMDAR2B antibody
    • NMDE2 antibody
    • NMDE2_HUMAN antibody
    • NME2 antibody
    • NR2B antibody
    • NR3 antibody
    see all

Anti-NMDAR2B antibody 画像

  • All lanes : Anti-NMDAR2B antibody (ab65783) at 1 µg/ml

    Lane 1 : Rat Hippocampus Tissue Lysate at 10 µg
    Lane 2 : Mouse Hippocampus Tissue Lysate (ab48631) at 10 µg
    Lane 3 : Human brain tissue lysate - total protein (ab29466) at 20 µg
    Lane 4 : Human brain hippocampus tissue lysate - total protein (ab30180) at 20 µg
    Lane 5 : Human brain amygdala tissue lysate - total protein at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 166 kDa
    Observed band size : 180 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 100 kDa,200 kDa,35 kDa,45 kDa,56 kDa,65 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes

    This blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab65783 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

  • All lanes : Anti-NMDAR2B antibody (ab65783) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Brain (Mouse) Tissue Lysate
    Lane 3 : Brain (Rat) Tissue Lysate
    Lane 4 : Hippocampus (Mouse) Tissue Lysate
    Lane 5 : Rat Hippocampus Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 166 kDa
    Observed band size : 180 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 35 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute
    NMDAR2B contains a number of potential phosphorylation and glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
  • ab65783 staining NR2B in SKNSH cells treated with GBR 12909 dihydrochloride (ab120607), by ICC/IF. Decrease in NR2B expression correlates with increased concentration of GBR 12909 dihydrochloride, as described in literature.
    The cells were incubated at 37°C for 10 minutes in media containing different concentrations of ab120607 (GBR 12909 dihydrochloride) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab65783 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
  • NMDAR2B was immunoprecipitated using 0.5mg Mouse Brain tissue lysate, 5µg of Rabbit polyclonal to NMDAR2B and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Mouse Brain tissue lysate lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab65783.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 180kDa; NMDAR2B
  • ICC/IF image of ab65783 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65783, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) PC12 cells at 5µg/ml.
  • NMDAR2B staining on mouse Cerebral cortex sections. The sections used came from animals perfused fixed with Paraformaldehyde 4%, in phosphate buffer 0.2M. Following postfixation in the same fixative overnight, the brains were cryoprotected in sucrose 30% overnight. Brains were then cut using a cryostat and the immunostainings were preformed using the ‘free floating’ technique.

    See Abreview

Anti-NMDAR2B antibody (ab65783) 使用論文

This product has been referenced in:
  • Cheng YC  et al. Knocking down of heat-shock protein 27 directs differentiation of functional glutamatergic neurons from placenta-derived multipotent cells. Sci Rep 6:30314 (2016). ICC/IF ; Human . Read more (PubMed: 27444754) »
  • Nath AR  et al. Cryoloading: introducing large molecules into live synaptosomes. Front Cell Neurosci 8:4 (2014). ICC/IF ; Chicken . Read more (PubMed: 24478628) »

See all 3 Publications for this product

Product Wall

We unfortunately do not have image for ab3856, however it has been tested with rat samples and is fully covered under Abpromise guarantee, http://www.abcam.com/content/abpromise-guarantee.



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Application Western blot
Loading amount 2 µg
Gel Running Conditions Reduced Denaturing (10)
Sample Xenopus laevis Cell lysate - whole cell (Tectal cell)
Specification Tectal cell
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 22°C
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Dr. WANHUA SHEN

Verified customer

投稿 Aug 01 2014

Thank you for your reply. I am still working on the alignments for some of your targets, but I have some antibodies that might interest you. We do not have any antibodies to the targets you listed which have been validated for use with zebrafis...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Brain (Cerebral Cortex))
Specification Brain (Cerebral Cortex)
Fixative Paraformaldehyde
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Dr. Sophie Pezet

Verified customer

投稿 Aug 26 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"