製品の概要

  • 製品名Anti-NMDAR2A (phospho Y1325) antibody
    NMDAR2A 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to NMDAR2A (phospho Y1325)
  • 特異性Specific for the ~180k NMDAR2A protein phosphorylated at Tyr1325 in Western blots. Immunolabelling is completely blocked by preadsorption with the phosphopeptide but not by the corresponding dephosphopeptide.
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Rat
    交差が予測される動物種: Cow, Dog
  • 免疫原

    Phosphopeptide corresponding to amino acid residues surrounding the phospho-Tyr1325 of NMDAR2A.

  • ポジティブ・コントロール
    • Rat hippocampal lysate

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 50% Glycerol, 100µg/ml BSA, 150mM Sodium chloride, 10mM HEPES, pH 7.5
  • Concentration information loading...
  • 精製度Protein A purified
  • 特記事項(精製)Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab106590 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 165 kDa.

ターゲット情報

  • 機能NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Activation requires binding of agonist to both types of subunits.
  • 配列類似性Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2A/GRIN2A subfamily.
  • 細胞内局在Cell membrane. Cell junction > synapse > postsynaptic cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • EPND antibody
    • FESD antibody
    • GluN2A antibody
    • Glutamate [NMDA] receptor subunit epsilon-1 antibody
    • Glutamate receptor antibody
    • Glutamate receptor ionotropic N methyl D aspartate 2A antibody
    • GRIN 2A antibody
    • GRIN2A antibody
    • hNR2A antibody
    • LKS antibody
    • N methyl D aspartate receptor channel, subunit epsilon 1 antibody
    • N Methyl D Aspartate Receptor Subtype 2A antibody
    • N methyl D aspartate receptor subunit 2A antibody
    • N-methyl D-aspartate receptor subtype 2A antibody
    • NMDA receptor subtype 2A antibody
    • NMDAR 2A antibody
    • NMDAR2A antibody
    • NMDE1_HUMAN antibody
    • NR2A antibody
    • OTTHUMP00000160135 antibody
    • OTTHUMP00000174531 antibody
    see all

Anti-NMDAR2A (phospho Y1325) antibody 画像

  • All lanes : Anti-NMDAR2A (phospho Y1325) antibody (ab106590) at 1/1000 dilution

    Lane 1 : Rat hippocampal lysate
    Lane 2 : Rat hippocampal lysate with preadsorption with the phospho-peptide


    Predicted band size : 165 kDa
    Observed band size : ~180 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-NMDAR2A (phospho Y1325) antibody (ab106590) at 1/500 dilution

    Lane 1 : Rat brain whole tissue lysate
    Lane 2 : Rat brain whole tissue lysate
    Lane 3 : Rat brain whole tissue lysate
    Lane 4 : Rat brain whole tissue lysate
    Lane 5 : Rat brain whole tissue lysate
    Lane 6 : Rat brain whole tissue lysate
    Lane 7 : Rat brain whole tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP Goat anti-rabbit IgG polyclonal at 1/2000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 165 kDa
    Observed band size : 180 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This image is courtesy of an Abreview submitted by Dr Xiaofan Wang

    See Abreview

Anti-NMDAR2A (phospho Y1325) antibody (ab106590) 使用論文

This product has been referenced in:

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Tissue lysate - whole (brain)
Loading amount 20 µg
Specification brain
Gel Running Conditions Reduced Denaturing (3-8% Acetate)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Xiaofan Wang

Verified customer

投稿 Apr 27 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"