製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to NMDAR2A
  • アプリケーション
    適用あり: WB, IPmore details
  • 種交差性
    交差種: Mouse, Rat, Monkey
  • 免疫原

    Fusion protein (Mouse) (C terminal last 200 amino acids).

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.05% Sodium Azide
    Constituents: 0.15M Sodium Chloride, 0.1M Tris-glycine. pH 7.4
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab14596 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 170 kDa.
IP Use at an assay dependent dilution.

ターゲット情報

  • 機能
    NMDA receptor subtype of glutamate-gated ion channels possesses high calcium permeability and voltage-dependent sensitivity to magnesium. Activation requires binding of agonist to both types of subunits.
  • 配列類似性
    Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR2A/GRIN2A subfamily.
  • 細胞内局在
    Cell membrane. Cell junction > synapse > postsynaptic cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • EPND antibody
    • FESD antibody
    • GluN2A antibody
    • Glutamate [NMDA] receptor subunit epsilon-1 antibody
    • Glutamate receptor antibody
    • Glutamate receptor ionotropic N methyl D aspartate 2A antibody
    • GRIN 2A antibody
    • GRIN2A antibody
    • hNR2A antibody
    • LKS antibody
    • N methyl D aspartate receptor channel, subunit epsilon 1 antibody
    • N Methyl D Aspartate Receptor Subtype 2A antibody
    • N methyl D aspartate receptor subunit 2A antibody
    • N-methyl D-aspartate receptor subtype 2A antibody
    • NMDA receptor subtype 2A antibody
    • NMDAR 2A antibody
    • NMDAR2A antibody
    • NMDE1_HUMAN antibody
    • NR2A antibody
    • OTTHUMP00000160135 antibody
    • OTTHUMP00000174531 antibody
    see all

画像

  • Anti-NMDAR2A antibody (ab14596) at 1/1000 dilution + whole cell lysate of synaptosomes of rat hippocampus at 15 µg

    Secondary
    HRP-conjugated goat anti-rabbit antibody at 1/10000 dilution
    Developed using the ECL technique

    Observed band size : 160 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

    This image is courtesy of an Abreview submitted by Dr Sophie Pezet

    See Abreview

参考文献

This product has been referenced in:
  • Liu E  et al. GSK-3ß deletion in dentate gyrus excitatory neuron impairs synaptic plasticity and memory. Sci Rep 7:5781 (2017). Read more (PubMed: 28720858) »
  • Gonzalez-Lozano MA  et al. Dynamics of the mouse brain cortical synaptic proteome during postnatal brain development. Sci Rep 6:35456 (2016). WB . Read more (PubMed: 27748445) »

See all 18 Publications for this product

レビューと Q&A

Application
Western blot
Sample
Chicken Cell lysate - whole cell (Homogenized chicken embryo cerebellum)
Gel Running Conditions
Non-reduced Denaturing (Non-reduced Denaturing (4-20% Mini-PROTEAN­ TGX™ Precast Gels))
Loading amount
25 µg
Specification
Homogenized chicken embryo cerebellum
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Mrs. Marthe Fjelldal

Verified customer

投稿 Mar 24 2017

Thank you for your message and for your patience.
It is difficult to find three secondary antibodies in three colors that woudl be suitable, but I hope the following will help. Please check the excitation and emission wavelenghts and spectra to en...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Cell lysate - other (synaptosomes of rat hippocampus)
Loading amount
15 µg
Specification
synaptosomes of rat hippocampus
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Sophie Pezet

Verified customer

投稿 Apr 08 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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