製品の概要

  • 製品名
    Anti-NMDAR1 (phospho S897) antibody
    NMDAR1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to NMDAR1 (phospho S897)
  • 由来種
    Rabbit
  • 特異性
    Detects endogenous levels of NMDAR1 only when phosphorylated at serine 897.
  • アプリケーション
    適用あり: WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic phosphopeptide derived from human NMDAR1 around the phosphorylation site of serine 897 (R-S-SP-K-D).

  • ポジティブ・コントロール
    • Human brain tissue.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab52184 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 105 kDa.
ELISA 1/10000.
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 機能
    NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. This protein plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors.
  • 配列類似性
    Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR1/GRIN1 subfamily.
  • 翻訳後修飾
    NMDA is probably regulated by C-terminal phosphorylation of an isoform of NR1 by PKC. Dephosphorylated on Ser-897 probably by protein phosphatase 2A (PPP2CB). Its phosphorylated state is influenced by the formation of the NMDAR-PPP2CB complex and the NMDAR channel activity.
  • 細胞内局在
    Cell membrane. Cell junction > synapse > postsynaptic cell membrane. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Enriched in post-synaptic plasma membrane and post-synaptic densities.
  • Information by UniProt
  • 参照データベース
  • 別名
    • GluN1 antibody
    • Glutamate [NMDA] receptor subunit zeta-1 antibody
    • Glutamate receptor ionotropic N methyl D aspartate 1 antibody
    • Glutamate receptor ionotropic, N-methyl-D aspartate, subunit 1 antibody
    • glutamate receptor ionotropic, NMDA 1 antibody
    • Grin1 antibody
    • MRD8 antibody
    • N methyl D aspartate receptor antibody
    • N methyl D aspartate receptor channel subunit zeta 1 antibody
    • N methyl D aspartate receptor subunit NR1 antibody
    • N-methyl-D-aspartate receptor subunit NR1 antibody
    • NMD-R1 antibody
    • NMDA 1 antibody
    • NMDA R1 antibody
    • NMDA receptor 1 antibody
    • NMDA1 antibody
    • NMDAR antibody
    • NMDZ1_HUMAN antibody
    • NR1 antibody
    see all

画像

  • Ab52184 at 1/50 dilution staining Human brain tissue with and without blocking peptide; paraffin embedded.

    Ab52184 at 1/50 dilution staining Human brain tissue with and without blocking peptide; paraffin embedded.
  • Western Blot analysis of LOVO cell lysate labeling NMDAR1 (phospho S897) with ab52184. The lane on the right is blocked with the phospho peptide.

参考文献

This product has been referenced in:
  • Lethbridge R  et al. Olfactory bulb glomerular NMDA receptors mediate olfactory nerve potentiation and odor preference learning in the neonate rat. PLoS One 7:e35024 (2012). IHC-FoFr ; Rat . Read more (PubMed: 22496886) »

See 1 Publication for this product

レビューと Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (brain, striatum)
Loading amount
20 µg
Specification
brain, striatum
Gel Running Conditions
Reduced Denaturing (4-20%)
Blocking step
SlimFast (soja-protein+milk) as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 23°C
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Abcam user community

Verified customer

投稿 May 11 2011

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (spinal cord)
Specification
spinal cord
Fixative
Paraformaldehyde
Permeabilization
Yes - nonionic detergents (Triton-X)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C
Username

Abcam user community

Verified customer

投稿 Jan 07 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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