製品の概要

  • 製品名Anti-NMDAR1 antibody
    NMDAR1 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to NMDAR1
  • 特異性This antibody is expected to recognise all three reported isoforms (NP_000823.4; NP_067544.1; NP_015566.1).
  • アプリケーション適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Mouse, Rat
    交差が予測される動物種: Chicken, Dog, Human, Xenopus laevis, Duck, Zebrafish
  • 免疫原

    Synthetic peptide:

    TQERVNNSNKKE

    , corresponding to internal sequence amino acids 486-497 from the internal region of Human NMDAR1 according to NP_000823.4; NP_067544.1; NP_015566.1

  • ポジティブ・コントロール
    • Rat brain lysates

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab77264 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA
WB
  • 追加情報ELISA: Use at an assay dependent dilution. Antibody detection limit dilution 1:1,000.
    WB: Use at a concentration of 2 - 4 µg/ml. Detects a band of approximately 23 and 105 kDa (predicted molecular weight: 105 kDa).

    The 23kDa band was strong in mouse brain lysates and faint in human brain lysates. The band was successfully blocked by incubation with the immunising peptide.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. This protein plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors.
    • 配列類似性Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR1/GRIN1 subfamily.
    • 翻訳後修飾NMDA is probably regulated by C-terminal phosphorylation of an isoform of NR1 by PKC. Dephosphorylated on Ser-897 probably by protein phosphatase 2A (PPP2CB). Its phosphorylated state is influenced by the formation of the NMDAR-PPP2CB complex and the NMDAR channel activity.
    • 細胞内局在Cell membrane. Cell junction > synapse > postsynaptic cell membrane. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Enriched in post-synaptic plasma membrane and post-synaptic densities.
    • Information by UniProt
    • 参照データベース
    • 別名
      • GluN1 antibody
      • Glutamate [NMDA] receptor subunit zeta-1 antibody
      • Glutamate receptor ionotropic N methyl D aspartate 1 antibody
      • Glutamate receptor ionotropic, N-methyl-D aspartate, subunit 1 antibody
      • glutamate receptor ionotropic, NMDA 1 antibody
      • Grin1 antibody
      • MRD8 antibody
      • N methyl D aspartate receptor antibody
      • N methyl D aspartate receptor channel subunit zeta 1 antibody
      • N methyl D aspartate receptor subunit NR1 antibody
      • N-methyl-D-aspartate receptor subunit NR1 antibody
      • NMD-R1 antibody
      • NMDA 1 antibody
      • NMDA R1 antibody
      • NMDA receptor 1 antibody
      • NMDA1 antibody
      • NMDAR antibody
      • NMDZ1_HUMAN antibody
      • NR1 antibody
      see all

    Anti-NMDAR1 antibody 画像

    • Anti-NMDAR1 antibody (ab77264) at 2 µg/ml + rat brain lysate in RIPA buffer at 35 µg
      Developed using the ECL technique

      Predicted band size : 105 kDa
      Observed band size : 105 kDa
      Additional bands at : 23 kDa. We are unsure as to the identity of these extra bands.

    Anti-NMDAR1 antibody (ab77264) 使用論文

    This product has been referenced in:
    • Cronican AA  et al. Genome-wide alteration of histone H3K9 acetylation pattern in mouse offspring prenatally exposed to arsenic. PLoS One 8:e53478 (2013). WB ; Mouse . Read more (PubMed: 23405071) »
    • Ghafari M  et al. NMDA-complexes linked to spatial memory performance in the Barnes maze in CD1 mice. Behav Brain Res : (2011). WB ; Mouse . Read more (PubMed: 21377497) »

    See all 2 Publications for this product

    Product Wall

    Thank you for your message and for your patience.
    It is difficult to find three secondary antibodies in three colors that woudl be suitable, but I hope the following will help. Please check the excitation and emission wavelenghts and spectra to en...

    Read More

    Thank you for contacting us. We do not have anti GluR1 and GLuR2 antibodies that can be used for Zebrafish. We unfortunately do not have information of GluR1 and GLuR2 protein sequence of Zebrafish so I am sorry we can’t check the homology. How...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"