Anti-NM23A 抗体 [EPR3036] (ab92327)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3036] to NM23A
- Suitable for: WB, IHC-P, Flow Cyt (Intra)
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-NM23A antibody [EPR3036]
NM23A 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR3036] to NM23A -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, Flow Cyt (Intra)more details
適用なし: IP -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human NM23A aa 1-100. The exact sequence is proprietary.
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ポジティブ・コントロール
- WB: HEK293T, MCF7, A375, HeLa and Jurkat whole cell lysate (ab7899) IHC-P: Human lung adenocarcinoma tissue
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR3036 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab92327の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 17 kDa.
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Overnight incubation is recommended.
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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特記事項 |
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WB
1/1000 - 1/5000. Predicted molecular weight: 17 kDa. |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Overnight incubation is recommended. |
Flow Cyt (Intra)
Use at an assay dependent concentration. |
ターゲット情報
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機能
Major role in the synthesis of nucleoside triphosphates other than ATP. Possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities. Involved in cell proliferation, differentiation and development, signal transduction, G protein-coupled receptor endocytosis, and gene expression. Required for neural development including neural patterning and cell fate determination. -
組織特異性
Isoform 1 is expressed in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, spleen and thymus. Expressed in lung carcinoma cell lines but not in normal lung tissues. Isoform 2 is ubiquitously expressed and its expression is also related to tumor differentiation. Isoform 3 is ubiquitously expressed. -
配列類似性
Belongs to the NDK family. -
細胞内局在
Cytoplasm. Nucleus. Cell-cycle dependent nuclear localization which can be induced by interaction with Epstein-barr viral proteins or by degradation of the SET complex by GzmA. - Information by UniProt
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参照データベース
- Entrez Gene: 4830 Human
- Omim: 156490 Human
- SwissProt: P15531 Human
- Unigene: 463456 Human
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別名
- AWD antibody
- AWD, drosophila, homolog of antibody
- GAAD antibody
see all
画像
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All lanes : Anti-NM23A antibody [EPR3036] (ab92327) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : NME1 knockout HEK293T cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 17 kDa
Observed band size: 17 kDaLanes 1-4: Merged signal (red and green). Green - ab92327 observed at 17 kDa. Red - loading control ab8245 observed at 36 kDa.
ab92327 Anti-NM23A antibody [EPR3036] was shown to specifically react with NM23A in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266215 (knockout cell lysate ab258075) was used. Wild-type and NM23A knockout samples were subjected to SDS-PAGE. ab92327 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling NM23A with unpurified ab92327 at 1/50 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti-rabbit IgG (Alexa Fluorr® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
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ab92327, at a dilution of 1/100, staining NM23A in formalin fixed, paraffin embedded Human lung adenocarcinoma tissue by Immunohistochemistry. Detection: DAB staining
Heat mediated antigen retrieval was performed via the pressure cooker method before commencing with IHC staining protocol.
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All lanes : Anti-NM23A antibody [EPR3036] (ab92327) at 1/5000 dilution
Lane 1 : MCF-7 cell lysate
Lane 2 : A375 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 17 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab92327 は 1 報の論文で使用されています。
- Yang L et al. Anti-tumor peptide SA12 inhibits metastasis of MDA-MB-231 and MCF-7 breast cancer cells via increasing expression of the tumor metastasis suppressor genes, CDH1, nm23-H1 and BRMS1. Exp Ther Med 20:1758-1763 (2020). PubMed: 32742405