製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to NLRX1
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rat, Cow, Dog
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 900 to the C-terminus of Human NLRX1.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following lysates: MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate; HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate; THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab91659 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 mg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 108 kDa).

ターゲット情報

  • 機能
    Participates in antiviral signaling. Acts as a negative regulator of MAVS-mediated antiviral responses, through the inhibition of the virus-induced RLH (RIG-like helicase)-MAVS interaction (PubMed:18200010). Has no inhibitory function on NF-Kappa-B and type 1 interferon signaling pathways, but enhances NF-Kappa-B and JUN N-terminal kinase dependent signaling through the production of reactive oxygen species (PubMed:18219313).
  • 組織特異性
    Ubiquitously expressed. Strongest expression in mammary gland, heart and muscle. Detected in HELA, 293T, THP-1, HL60, RAJI, and JURKAT cell lines (at protein level).
  • 配列類似性
    Belongs to the NLRP family.
    Contains 7 LRR (leucine-rich) repeats.
    Contains 1 NACHT domain.
  • 細胞内局在
    Mitochondrion outer membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Caterpiller protein 11.3 antibody
    • CLR11.3 antibody
    • DLNB26 antibody
    • FLJ21478 antibody
    • MGC131937 antibody
    • MGC21025 antibody
    • NLR family member X1 antibody
    • NLR family, X1 antibody
    • Nlrx1 antibody
    • NLRX1_HUMAN antibody
    • NOD-like receptor X1 antibody
    • NOD26 antibody
    • NOD5 antibody
    • NOD9 antibody
    • Nucleotide-binding oligomerization domain protein 26 antibody
    • Nucleotide-binding oligomerization domain protein 5 antibody
    • Nucleotide-binding oligomerization domain protein 9 antibody
    • nucleotide-binding oligomerization domain, leucine rich repeat containing X1 antibody
    • Protein Caterpiller 11.3 antibody
    see all

画像

  • All lanes : Anti-NLRX1 antibody (ab91659) at 1 µg/ml

    Lane 1 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 3 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 108 kDa
    Observed band size : 110 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 60 kDa,75 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes

プロトコール

参考文献

ab91659 has not yet been referenced specifically in any publications.

レビューと Q&A

There are currently no Customer reviews or Questions for ab91659.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録