Anti-NLRX1 抗体 (ab105412)
Key features and details
- Rabbit polyclonal to NLRX1
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Rat
- Isotype: IgG
製品の概要
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製品名
Anti-NLRX1 antibody
NLRX1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to NLRX1 -
由来種
Rabbit -
特異性
At least two isoforms of NLRX1 are known to exist; ab105412 will recognize both. This antibody is predicted to not crossreact with other NLRP family members. -
アプリケーション
適用あり: ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Rat -
免疫原
A 14 amino acid peptide near the amino terminus of Human NLRX1 (NP_078894).
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ポジティブ・コントロール
- Rat spleen tissue lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab105412の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 20 µg/ml.
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 108 kDa.
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IHC-P |
Use a concentration of 10 µg/ml.
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特記事項 |
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ICC/IF
Use a concentration of 20 µg/ml. |
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 108 kDa. |
IHC-P
Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
Participates in antiviral signaling. Acts as a negative regulator of MAVS-mediated antiviral responses, through the inhibition of the virus-induced RLH (RIG-like helicase)-MAVS interaction (PubMed:18200010). Has no inhibitory function on NF-Kappa-B and type 1 interferon signaling pathways, but enhances NF-Kappa-B and JUN N-terminal kinase dependent signaling through the production of reactive oxygen species (PubMed:18219313). -
組織特異性
Ubiquitously expressed. Strongest expression in mammary gland, heart and muscle. Detected in HELA, 293T, THP-1, HL60, RAJI, and JURKAT cell lines (at protein level). -
配列類似性
Belongs to the NLRP family.
Contains 7 LRR (leucine-rich) repeats.
Contains 1 NACHT domain. -
細胞内局在
Mitochondrion outer membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 315599 Rat
- SwissProt: Q5FVQ8 Rat
- Unigene: 12444 Rat
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別名
- Caterpiller protein 11.3 antibody
- CLR11.3 antibody
- DLNB26 antibody
see all
画像
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Immunofluorescence of NLRX1 in rat spleen tissue with ab105412 at 20 ug/mL.
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Lane 1 : Anti-NLRX1 antibody (ab105412) at 1 µg/ml
Lane 2 : Anti-NLRX1 antibody (ab105412) at 2 µg/ml
All lanes : rat spleen tissue cell lysate
Predicted band size: 108 kDa -
Immunohistochemistry of NLRX1 in rat spleen tissue with ab105412 at 10 µg/ml.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab105412 は 3 報の論文で使用されています。
- He B et al. Long noncoding RNA NKILA transferred by astrocyte-derived extracellular vesicles protects against neuronal injury by upregulating NLRX1 through binding to mir-195 in traumatic brain injury. Aging (Albany NY) 13:8127-8145 (2021). PubMed: 33686956
- Peng L et al. DJ-1 exerts anti-inflammatory effects and regulates NLRX1-TRAF6 via SHP-1 in stroke. J Neuroinflammation 17:81 (2020). PubMed: 32151250
- Luo X et al. HPV16 drives cancer immune escape via NLRX1-mediated degradation of STING. J Clin Invest 130:1635-1652 (2020). PubMed: 31874109