製品の概要

  • 製品名Anti-nkx6.3 antibody
    nkx6.3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to nkx6.3
  • 特異性ab69946 detects endogenous levels of total nkx6.3 protein.
  • アプリケーション適用あり: WB, ELISA, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic peptide derived from an internal sequence of human nkx6.3

  • ポジティブ・コントロール
    • HUVEC cells and cell extracts from K562 and COS7 cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab69946 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 34 kDa (predicted molecular weight: 29 kDa).
ELISA 1/5000.
ICC/IF 1/500 - 1/1000.

ターゲット情報

Anti-nkx6.3 antibody 画像

  • ab69946 at 1/500 dilution staining nkx6.3 in HUVEC cells by Immunofluorescence, in the absence or presence of the immunising peptide.
  • All lanes : Anti-nkx6.3 antibody (ab69946) at 1/500 dilution

    Lane 1 : K562 cell extract
    Lane 2 : COS7 cell extract
    Lane 3 : K562 cell extract with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 29 kDa
    Observed band size : 34 kDa (why is the actual band size different from the predicted?)

Anti-nkx6.3 antibody (ab69946) 使用論文

ab69946 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Blocking step 0.3M glycine, donkey serum, 1% BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Sample Mouse Cell (adult stomach, embryonic brain)
Specification adult stomach, embryonic brain
Permeabilization Yes - 0.5% Triton-X 100
Fixative Paraformaldehyde
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投稿 Sep 17 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"