Anti-Nkx2.5 抗体 (ab106923)
Key features and details
- Goat polyclonal to Nkx2.5
- Suitable for: WB, ICC, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Nkx2.5 antibody
Nkx2.5 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to Nkx2.5 -
由来種
Goat -
特異性
ab106923 is expected to recognize all three reported isoforms (NP_004378.1; NP_001159647.1; NP_001159648.1).
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アプリケーション
適用あり: WB, ICC, IHC-Pmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide within Human Nkx2.5 aa 50-150 (internal sequence). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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ポジティブ・コントロール
- WB: Mouse heart lysate; ICC: U-2 OS cells. IHC: Human heart.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab106923 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab106923の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
Use a concentration of 1 - 0.5 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 35 kDa).
A 1 hour primary incubation is recommended for this product. Abcam recommends using 3% milk as the blocking agent. |
ICC |
Use a concentration of 10 µg/ml.
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IHC-P |
Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Only suitable for human, not mouse samples. |
特記事項 |
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WB
Use a concentration of 1 - 0.5 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 35 kDa). A 1 hour primary incubation is recommended for this product. Abcam recommends using 3% milk as the blocking agent. |
ICC
Use a concentration of 10 µg/ml. |
IHC-P
Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Only suitable for human, not mouse samples. |
ターゲット情報
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機能
Implicated in commitment to and/or differentiation of the myocardial lineage. Acts as a transcriptional activator of ANF in cooperation with GATA4 (By similarity). It is transcriptionally controlled by PBX1 and acts as a transcriptional repressor of CDKN2B (By similarity). It is required for spleen development. -
組織特異性
Expressed only in the heart. -
関連疾患
Atrial septal defect 7, with or without atrioventricular conduction defects
Tetralogy of Fallot
Conotruncal heart malformations
Hypothyroidism, congenital, non-goitrous, 5
Ventricular septal defect 3
Hypoplastic left heart syndrome 2
Asplenia, isolated congenital -
配列類似性
Belongs to the NK-2 homeobox family.
Contains 1 homeobox DNA-binding domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 1482 Human
- Entrez Gene: 18091 Mouse
- Omim: 600584 Human
- SwissProt: P52952 Human
- SwissProt: P42582 Mouse
- Unigene: 54473 Human
- Unigene: 41974 Mouse
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別名
- NKX2.5, mouse, homolog of antibody
- Cardiac-specific homeobox 1 antibody
- Cardiac-specific homeobox antibody
see all
画像
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All lanes : Anti-Nkx2.5 antibody (ab106923) at 1 µg/ml
Lane 1 : Human heart lysate in RIPA buffer
Lane 2 : Mouse heart lysate in RIPA buffer
Lane 3 : Rat heart lysate in RIPA buffer
Lysates/proteins at 35 µg per lane.
Predicted band size: 35 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?Detected by chemiluminescence.
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Immunocytochemistry analysis of paraformaldehyde fixed U-2 OS (human bone osteosarcoma epithelial cell line) cells, permeabilized with 0.15% Triton. Primary incubation for 1 hour at 10 µg/mL followed by Alexa Fluor® 488 secondary antibody at 2 µg/mL. The nuclear stain is DAPI (blue).
Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody (2 µg/mL).
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Immunohistochemical analysis of human heart tissue stained for Nkx2.5 using ab106923 at 4µg/ml. Positive cytoplasmic staining shown.
データシートおよび資料
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Datasheet download
参考文献 (6)
ab106923 は 6 報の論文で使用されています。
- Zhu D et al. Minimally invasive delivery of therapeutic agents by hydrogel injection into the pericardial cavity for cardiac repair. Nat Commun 12:1412 (2021). PubMed: 33658506
- Yu B et al. Circular RNA hsa_circ_105039 promotes cardiomyocyte differentiation by sponging miR‑17 to regulate cyclinD2 expression. Mol Med Rep 24:N/A (2021). PubMed: 34664684
- Chatterjee S & Chakravarti A A gene regulatory network explains RET-EDNRB epistasis in Hirschsprung disease. Hum Mol Genet 28:3137-3147 (2019). PubMed: 31313802
- Chen J et al. Nkx2.5 insufficiency leads to atrial electrical remodeling through Wnt signaling in HL-1 cells. Exp Ther Med 18:4631-4636 (2019). PubMed: 31798700
- Liu H et al. Resveratrol Enhances Cardiomyocyte Differentiation of Human Induced Pluripotent Stem Cells through Inhibiting Canonical WNT Signal Pathway and Enhancing Serum Response Factor-miR-1 Axis. Stem Cells Int 2016:2524092 (2016). PubMed: 26798354
- Mohammadi A et al. Generation of Rat Embryonic Germ Cells via Inhibition of TGFß and MEK Pathways. Cell J 17:288-95 (2015). Rat . PubMed: 26199907